Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egress

Autor: Maria Harkiolaki, Marisa L. Martin-Fernandez, Mohamed A. Koronfel, Anna-Sophia Krebs, Michael L. Knight, Andy G. Howe, Tao Ni, Luiza Mendonça, Yuewen Sheng, Peijun Zhang, Laura C. Zanetti-Domingues, Marta Szynkiewicz, Dapeng Sun, Long Chen, Julika Radecke, James B Gilchrist, Ilias Kounatidis, Vivian D Li, William James, Benji C. Bateman
Přispěvatelé: Mendonça, Luiza [0000-0002-9210-9139], Howe, Andrew [0000-0003-2187-7672], Gilchrist, James B [0000-0002-9814-4285], Sheng, Yuewen [0000-0003-3067-9520], Sun, Dapeng [0000-0003-0220-8844], Knight, Michael L [0000-0002-8780-1630], Zanetti-Domingues, Laura C [0000-0003-0514-3185], Bateman, Benji [0000-0003-4580-4662], Krebs, Anna-Sophia [0000-0001-6244-9899], Chen, Long [0000-0001-5595-3026], Radecke, Julika [0000-0002-5815-5537], Li, Vivian D [0000-0002-1923-0231], Ni, Tao [0000-0001-7268-0306], Koronfel, Mohamed A [0000-0001-7156-7176], Harkiolaki, Maria [0000-0001-8091-9057], Martin-Fernandez, Marisa L [0000-0001-5496-6300], James, William [0000-0002-2506-1198], Zhang, Peijun [0000-0003-1803-691X], Apollo - University of Cambridge Repository, Gilchrist, James B. [0000-0002-9814-4285], Knight, Michael L. [0000-0002-8780-1630], Zanetti-Domingues, Laura C. [0000-0003-0514-3185], Li, Vivian D. [0000-0002-1923-0231], Koronfel, Mohamed A. [0000-0001-7156-7176], Martin-Fernandez, Marisa L. [0000-0001-5496-6300]
Rok vydání: 2021
Předmět:
Electron Microscope Tomography
viruses
Cell
General Physics and Astronomy
Virus Replication
14
0302 clinical medicine
Chlorocebus aethiops
skin and connective tissue diseases
health care economics and organizations
Virus Release
0303 health sciences
Multidisciplinary
cryoFIB/SEM
Virus structures
humanities
egress
Cell biology
cryoEM
soft X-ray
medicine.anatomical_structure
631/326/596/2148
9
cryoET
Pathogens
631/535/1258/1260
Correlative
101
Science
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
education
Context (language use)
Biology
Article
General Biochemistry
Genetics and Molecular Biology
Virus
03 medical and health sciences
medicine
subtomogram averaging
Animals
Humans
Pandemics
Vero Cells
631/326/596/4130
030304 developmental biology
631/326/421
SARS-CoV-2
Virus Assembly
101/28
Cryoelectron Microscopy
fungi
COVID-19
spike
General Chemistry
body regions
cryo-tomography
Viral replication
Vero cell
Cryoelectron tomography
030217 neurology & neurosurgery
Zdroj: Nature Communications
Nature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
Research Square
article-version (status) pre
article-version (number) 1
ISSN: 2041-1723
DOI: 10.1038/s41467-021-24887-y
Popis: Funder: Medical Research Council
Since the outbreak of the SARS-CoV-2 pandemic, there have been intense structural studies on purified viral components and inactivated viruses. However, structural and ultrastructural evidence on how the SARS-CoV-2 infection progresses in the native cellular context is scarce, and there is a lack of comprehensive knowledge on the SARS-CoV-2 replicative cycle. To correlate cytopathic events induced by SARS-CoV-2 with virus replication processes in frozen-hydrated cells, we established a unique multi-modal, multi-scale cryo-correlative platform to image SARS-CoV-2 infection in Vero cells. This platform combines serial cryoFIB/SEM volume imaging and soft X-ray cryo-tomography with cell lamellae-based cryo-electron tomography (cryoET) and subtomogram averaging. Here we report critical SARS-CoV-2 structural events - e.g. viral RNA transport portals, virus assembly intermediates, virus egress pathway, and native virus spike structures, in the context of whole-cell volumes revealing drastic cytppathic changes. This integrated approach allows a holistic view of SARS-CoV-2 infection, from the whole cell to individual molecules.
Databáze: OpenAIRE
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