Expanding Actin Rings Zipper the Mouse Embryo for Blastocyst Formation

Autor: Jennifer Zenker, Hui Yi Grace Lim, Melanie D. White, Maté Biro, Maxime Gasnier, Stephanie Bissiere, Nicolas Plachta, Yanina Daniela Alvarez
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
LIVE IMAGING
TIGHT JUNCTIONS
Cell Communication
BLASTOCYST
Microtubules
ACTIN DYNAMICS
purl.org/becyt/ford/1 [https]
EPITHELIA
Mice
Myosin
RNA
Small Interfering
Tight junction
CORTICAL FLOW
Embryo
Cell biology
MICROTUBULES
medicine.anatomical_structure
embryonic structures
MAMMALIAN DEVELOPMENT
Female
PREIMPLANTATION MOUSE EMBRYO
CIENCIAS NATURALES Y EXACTAS
animal structures
Green Fluorescent Proteins
Embryonic Development
macromolecular substances
Biology
Morula
General Biochemistry
Genetics and Molecular Biology
Tight Junctions
Adherens junction
Ciencias Biológicas
03 medical and health sciences
Imaging
Three-Dimensional
Biología Celular
Microbiología
Microtubule
medicine
Animals
Blastocyst
purl.org/becyt/ford/1.6 [https]
Actin
Myosin Type II
Embryogenesis
Embryo
Mammalian
Actins
Mice
Inbred C57BL
Cytoskeletal Proteins
030104 developmental biology
MORPHOGENESIS
Zdroj: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
Popis: Transformation from morula to blastocyst is a defining event of preimplantation embryo development. During this transition, the embryo must establish a paracellular permeability barrier to enable expansion of the blastocyst cavity. Here, using live imaging of mouse embryos, we reveal an actin-zippering mechanism driving this embryo sealing. Preceding blastocyst stage, a cortical F-actin ring assembles at the apical pole of the embryo's outer cells. The ring structure forms when cortical actin flows encounter a network of polar microtubules that exclude F-actin. Unlike stereotypical actin rings, the actin rings of the mouse embryo are not contractile, but instead, they expand to the cell-cell junctions. Here, they couple to the junctions by recruiting and stabilizing adherens and tight junction components. Coupling of the actin rings triggers localized myosin II accumulation, and it initiates a tension-dependent zippering mechanism along the junctions that is required to seal the embryo for blastocyst formation. Expanding actin rings zipper the mouse embryo to seal it and allow formation of the blastocyst cavity. Fil: Zenker, Jennifer. Institute Of Molecular And Cell Biology, A-star, Singapore; Fil: White, Melanie D.. Institute Of Molecular And Cell Biology, A-star, Singapore; Fil: Gasnier, Maxime. Institute Of Molecular And Cell Biology, A-star, Singapore; Fil: Alvarez, Yanina Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina Fil: Lim, Hui Yi Grace. Institute Of Molecular And Cell Biology, A-star, Singapore; Fil: Bissiere, Stephanie. Institute Of Molecular And Cell Biology, A-star, Singapore; Fil: Biro, Maté. Institute Of Molecular And Cell Biology, A-star, Singapore; Fil: Plachta, Nicolas. Institute Of Molecular And Cell Biology, A-star, Singapore
Databáze: OpenAIRE
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