Expanding Actin Rings Zipper the Mouse Embryo for Blastocyst Formation
Autor: | Jennifer Zenker, Hui Yi Grace Lim, Melanie D. White, Maté Biro, Maxime Gasnier, Stephanie Bissiere, Nicolas Plachta, Yanina Daniela Alvarez |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
LIVE IMAGING TIGHT JUNCTIONS Cell Communication BLASTOCYST Microtubules ACTIN DYNAMICS purl.org/becyt/ford/1 [https] EPITHELIA Mice Myosin RNA Small Interfering Tight junction CORTICAL FLOW Embryo Cell biology MICROTUBULES medicine.anatomical_structure embryonic structures MAMMALIAN DEVELOPMENT Female PREIMPLANTATION MOUSE EMBRYO CIENCIAS NATURALES Y EXACTAS animal structures Green Fluorescent Proteins Embryonic Development macromolecular substances Biology Morula General Biochemistry Genetics and Molecular Biology Tight Junctions Adherens junction Ciencias Biológicas 03 medical and health sciences Imaging Three-Dimensional Biología Celular Microbiología Microtubule medicine Animals Blastocyst purl.org/becyt/ford/1.6 [https] Actin Myosin Type II Embryogenesis Embryo Mammalian Actins Mice Inbred C57BL Cytoskeletal Proteins 030104 developmental biology MORPHOGENESIS |
Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
Popis: | Transformation from morula to blastocyst is a defining event of preimplantation embryo development. During this transition, the embryo must establish a paracellular permeability barrier to enable expansion of the blastocyst cavity. Here, using live imaging of mouse embryos, we reveal an actin-zippering mechanism driving this embryo sealing. Preceding blastocyst stage, a cortical F-actin ring assembles at the apical pole of the embryo's outer cells. The ring structure forms when cortical actin flows encounter a network of polar microtubules that exclude F-actin. Unlike stereotypical actin rings, the actin rings of the mouse embryo are not contractile, but instead, they expand to the cell-cell junctions. Here, they couple to the junctions by recruiting and stabilizing adherens and tight junction components. Coupling of the actin rings triggers localized myosin II accumulation, and it initiates a tension-dependent zippering mechanism along the junctions that is required to seal the embryo for blastocyst formation. Expanding actin rings zipper the mouse embryo to seal it and allow formation of the blastocyst cavity. Fil: Zenker, Jennifer. Institute Of Molecular And Cell Biology, A-star, Singapore; Fil: White, Melanie D.. Institute Of Molecular And Cell Biology, A-star, Singapore; Fil: Gasnier, Maxime. Institute Of Molecular And Cell Biology, A-star, Singapore; Fil: Alvarez, Yanina Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina Fil: Lim, Hui Yi Grace. Institute Of Molecular And Cell Biology, A-star, Singapore; Fil: Bissiere, Stephanie. Institute Of Molecular And Cell Biology, A-star, Singapore; Fil: Biro, Maté. Institute Of Molecular And Cell Biology, A-star, Singapore; Fil: Plachta, Nicolas. Institute Of Molecular And Cell Biology, A-star, Singapore |
Databáze: | OpenAIRE |
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