Mesenchymal Stem Cells Induce a Fibrolytic Phenotype By Regulating mmu-miR-6769b-5p Expression in Macrophages
| Autor: | Koichi Fujisawa, Isao Sakaida, Yutaka Suehiro, Naoki Yamamoto, Toshihiko Matsumoto, Taro Takami, Maiko Nishi, Takahiro Yamasaki |
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| Rok vydání: | 2020 |
| Předmět: |
Lipopolysaccharides
Male 0301 basic medicine medicine.medical_treatment Down-Regulation Economic shortage Liver transplantation Biology Extracellular Vesicles 03 medical and health sciences 0302 clinical medicine Fibrosis medicine Animals Radical treatment Fibrin Macrophages ATF4 Mesenchymal stem cell Mesenchymal Stem Cells Cell Biology Hematology medicine.disease Decompensated cirrhosis Activating Transcription Factor 4 Phenotype Coculture Techniques Up-Regulation Mice Inbred C57BL MicroRNAs 030104 developmental biology Gene Expression Regulation Matrix Metalloproteinase 9 Cancer research Cytokines Inflammation Mediators 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Stem Cells and Development. 29:1457-1466 |
| ISSN: | 1557-8534 1547-3287 |
| Popis: | Liver transplantation is the only radical treatment for decompensated cirrhosis, but its use is limited owing to a shortage of donors; hence, there is an urgent need for new treatments. Previously, we developed a liver-regeneration therapy using autologous bone marrow-derived mesenchymal stem cells (BMSCs), which is under clinical investigation. Cell-cell interactions between BMSCs and macrophages (Mφs) participate in the improvement of liver function and alleviation of liver fibrosis, although the associated mechanisms have not been elucidated. Therefore, in this study, we investigated phenotypic changes in Mφs caused by interactions with BMSCs, as well as the underlying mechanisms. Co-culturing lipopolysaccharide (LPS)-stimulated murine bone marrow-derived Mφs (BMDMs) with BMSCs substantially upregulated matrix metalloproteinase 9 ( |
| Databáze: | OpenAIRE |
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