Estradiol promotes spine growth and synapse formation without affecting pre-established networks
| Autor: | Pablo Mendez, Dominique Muller, Luis M. Garcia-Segura |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Male
musculoskeletal diseases Dendritic spine Cognitive Neuroscience Synaptogenesis Hippocampus Estrogen receptor In Vitro Techniques Hippocampal formation Culture Media Serum-Free 03 medical and health sciences 0302 clinical medicine Animals Nerve Net/drug effects/ultrastructure Synapse formation CA1 Region Hippocampal 030304 developmental biology 0303 health sciences Microscopy Confocal Synapses/drug effects Estradiol Chemistry CA1 Region Hippocampal/drug effects/ultrastructure Culture Media Serum-Free/pharmacology Dendrites musculoskeletal system ddc:616.8 Rats 3. Good health Spine (zoology) Dendrites/drug effects/ultrastructure Estradiol/pharmacology Animals Newborn Synapses Microscopy Electron Scanning Female Nerve Net Neuroscience 030217 neurology & neurosurgery Hormone |
| Zdroj: | Hippocampus Hippocampus, Vol. 21, No 12 (2011) pp. 1263-7 Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1050-9631 |
| Popis: | Estrogens regulate dendritic spine density, but the mechanism and significance of this effect for brain networks remain unknown. We used repetitive imaging over several days to investigate how 17β-estradiol affected the turnover and long-term behavior of dendritic spines in CA1 cells of hippocampal slice cultures. We find that 17β-estradiol and serum in the culture medium tightly regulated spine density by promoting an increase in the rate of new spine formation and their transformation into synapses, without affecting spine elimination or stability. New spines formed during a transient 17β-estradiol application were preferentially eliminated upon removal of the hormone, in contrast with pre-existing spines that remained unaffected. Our results reveal that 17β-estradiol transiently regulates the complexity of hippocampal circuits without causing major alterations of pre-existing networks. © 2010 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
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