Transfer of protein antigens into milk after intravenous injection into lactating mice
| Autor: | D. G. Hanson, M. K. Walsh, W A Walker, Paul Harmatz, Ronald E. Kleinman, Kurt J. Bloch |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
medicine.medical_specialty
Globulin Physiology Ratón Ovalbumin Endocrinology Diabetes and Metabolism Lactoglobulins Iodine Radioisotopes Mice Antigen Pregnancy Physiology (medical) Internal medicine Lactation medicine Animals Bovine serum albumin Antigens Serum Albumin Radio-Iodinated biology Chemistry Albumin Biological materials medicine.anatomical_structure Endocrinology Milk biology.protein Female Dietary Proteins gamma-Globulins Food Hypersensitivity |
| Zdroj: | The American journal of physiology. 251(2 Pt 1) |
| ISSN: | 0002-9513 |
| Popis: | We investigated the transfer of bovine serum 125I-albumin (125I-BSA), bovine 125I-gamma-globulin (125I-BGG), 125I-ovalbumin (125I-OVA), and 125I-beta-lactoglobulin (125I-BLG) from the blood into the milk of lactating mice. Equal amounts (by weight) of the radiolabeled proteins were injected intravenously into mice 1 wk postpartum. Total radioactivity, trichloroacetic acid-precipitable radioactivity, and specifically immunoprecipitable radioactivity were measured in serum, mammary gland homogenate, and milk. Clearance of immunoreactive OVA (iOVA) and iBLG from the circulation was more rapid than iBSA and iBGG. The radioactivity in mammary tissue associated with BSA and BGG was greater than 70% immunoprecipitable throughout the 4-h test interval; 125I-OVA and 125I-BLG were less than 12% precipitable 1 and 4 h after injection. In milk obtained at 4 h, there was an approximately 10-fold greater accumulation of iBSA or iBGG than of iOVA or iBLG. These experiments demonstrate that protein antigens differ in their ability to transfer from maternal circulation into milk. The transfer into milk appeared to be in proportion to persistence of the antigens in the maternal circulation. |
| Databáze: | OpenAIRE |
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