Developmentally Regulated Expression of CGRP in the Mouse Olfactory Pathway
| Autor: | M. Vitadello, S. Denis-Donini, B. Chini |
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| Rok vydání: | 1993 |
| Předmět: |
Olfactory system
Aging Neurofilament Calcitonin Gene-Related Peptide Neuropeptide Mice Inbred Strains In situ hybridization Calcitonin gene-related peptide Biology Mice medicine Animals RNA Messenger In Situ Hybridization Olfactory receptor General Neuroscience Olfactory Pathways RNA Probes Immunohistochemistry Olfactory bulb Cell biology medicine.anatomical_structure Animals Newborn nervous system Olfactory ensheathing glia Neuroscience |
| Zdroj: | European Journal of Neuroscience. 5:648-656 |
| ISSN: | 1460-9568 0953-816X |
| DOI: | 10.1111/j.1460-9568.1993.tb00530.x |
| Popis: | The pattern of expression of the neuropeptide CGRP and its encoding mRNA has been determined by immunohistochemistry and in situ hybridization in the mouse olfactory pathway during development. Specific CGRP transcripts are first detected at E13 followed by the appearance of the peptide at E15. Both peptide and transcript are present until birth; their expression then appears to be down-regulated since postnatally the peptide is only observed in some olfactory receptor neurons. A monoclonal antibody that specifically recognizes the neurofilament subunit NF-M has been used in order to identify olfactory and trigeminal axons. Our results demonstrate that CGRP is expressed in olfactory neurons and their axons during development, thus supporting further its role as a differentiation factor during olfactory bulb ontogenesis. |
| Databáze: | OpenAIRE |
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