Igh-6(-/-) (B-cell-deficient) mice fail to mount solid acquired resistance to oral challenge with virulent Salmonella enterica serovar typhimurium and show impaired Th1 T-cell responses to Salmonella antigens
| Autor: | Cameron P. Simmons, G. Dougan, Ray Fowler, Pietro Mastroeni, C E Hormaeche |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
Salmonella typhimurium Interleukin 2 Cellular immunity Salmonella T cell Immunology Administration Oral chemical and pharmacologic phenomena Biology Vaccines Attenuated medicine.disease_cause Microbiology Interferon-gamma Mice Immune system hemic and lymphatic diseases medicine Animals Mice Knockout Antigens Bacterial B-Lymphocytes Salmonella Infections Animal Genes Immunoglobulin Virulence Immunization Passive Th1 Cells Acquired immune system biology.organism_classification Virology Mice Inbred C57BL Bacterial vaccine Infectious Diseases medicine.anatomical_structure Salmonella enterica Bacterial Vaccines Microbial Immunity and Vaccines Interleukin-2 Female Immunization Parasitology Immunoglobulin Heavy Chains medicine.drug |
| Zdroj: | Scopus-Elsevier |
| DOI: | 10.1128/iai.68.1.46-53.2000 |
| Popis: | In the present study we evaluated the role of B cells in acquired immunity toSalmonellainfection by using gene-targeted B-cell-deficient innately susceptible mice on a C57BL/6 background (Igh-6−/−).Igh-6−/−mice immunized with a live, attenuatedaroA Salmonella entericaserovar Typhimurium vaccine strain showed impaired long-term acquired resistance against the virulent serovar Typhimurium strain C5.Igh-6−/−mice were able to control a primary infection and to clear the inoculum from the reticuloendothelial system. However,Igh-6−/−mice, unlikeIgh-6+/+C57BL/6 controls, did not survive an oral challenge with strain C5 at 4 months after vaccination. Transfer of immune serum did not restore resistance inIgh-6−/−mice. Total splenocytes and purified CD4+T cells obtained fromIgh-6−/−mice 4 months after vaccination showed reduced ability to release Th1-type cytokines (interleukin 2 and gamma interferon) upon in vitro restimulation with serovar Typhimurium soluble cell extracts compared to cells obtained fromIgh-6+/+C57BL/6 control mice. Therefore, the impaired resistance to oral challenge with virulent serovar Typhimurium observed in B-cell-deficient mice, which cannot be restored by passive transfer ofSalmonella-immune serum, may be in part due to a reduced serovar Typhimurium-specific T-cell response following primary immunization. |
| Databáze: | OpenAIRE |
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