In vitro profiling of the potential endocrine disrupting activities affecting steroid and aryl hydrocarbon receptors of compounds and mixtures prevalent in human drinking water resources

Autor: Doan Tq, Marc Muller, Scippo Ml, Lisa Connolly, Nott K, Ahmed Igout
Rok vydání: 2020
Předmět:
Bisphenol A
Environmental Engineering
Polychlorinated Dibenzodioxins
Bisphenol
Health
Toxicology and Mutagenesis
0208 environmental biotechnology
02 engineering and technology
010501 environmental sciences
Endocrine Disruptors
01 natural sciences
chemistry.chemical_compound
Transactivation
SDG 3 - Good Health and Well-being
Phenols
Genes
Reporter
Basic Helix-Loop-Helix Transcription Factors
Benzo(a)pyrene
Environmental Chemistry
Animals
Humans
Benzhydryl Compounds
Pesticides
Polycyclic Aromatic Hydrocarbons
Receptor
0105 earth and related environmental sciences
Fluoranthene
biology
Drinking Water
Public Health
Environmental and Occupational Health
Estrogens
General Medicine
General Chemistry
Aryl hydrocarbon receptor
Pollution
6. Clean water
3. Good health
020801 environmental engineering
Rats
chemistry
Biochemistry
Receptors
Aryl Hydrocarbon
13. Climate action
biology.protein
Water Resources
Pyrene
Water Pollutants
Chemical
Zdroj: Chemosphere
Connolly, L 2020, ' In vitro profiling of the potential endocrine disrupting activities affecting steroid and aryl hydrocarbon receptors of compounds and mixtures prevalent in human drinking water resources ', Chemosphere, vol. 258 . https://doi.org/10.1016/j.chemosphere.2020.127332
ISSN: 1879-1298
DOI: 10.1016/j.chemosphere.2020.127332
Popis: Prioritizing chemicals posing threats to drinking water resources is crucial for legislation considering the cost of water treatment, remediation, and monitoring. We profiled in vitro potential endocrine disrupting activities (both agonistic and antagonistic) of 18 contaminants most prevalent in Walloon raw water resources intended for drinking water production, including several compound groups: pesticides, perfluorinated compounds, polycyclic aromatic hydrocarbons, a corrosion inhibitor, and bisphenol A. Mixtures thereof relevant for human realistic exposure were also investigated. Seven luciferase reporter gene cell lines were used i.e. three (human and rat) responsive to dioxins through the aryl hydrocarbon receptor (AhR) and four (human) responsive to steroids through the estrogen (ER), androgen (AR), progesterone (PR), and glucocorticoid (GR) receptors. Among the 18 compounds, ten caused at least one response in at least one receptor. Specifically, chlorpyrifos, bisphenol A, fluoranthene, phenanthrene, and benzo [a]pyrene displayed significant activities on several receptors. Bisphenol A agonized ER, but abolished the cells’ response to androgen and progesterone. While fluoranthene and phenanthrene strongly reduced human AhR and AR transactivation, benzo [a]pyrene strongly activated AhR and ER, but inhibited GR and AR. In human breast cancer cells, benzo [a]pyrene dramatically activated AhR, inducing a 10-fold higher response than 2,3,7,8-tetrachlorodibenzodioxin (TCDD) at concentrations possibly found realistically in human blood. The mixture of the 18 compounds exerted both ER and rat AhR agonism, with the main contribution being from benzo [a]pyrene or its combination with bisphenol A. Moreover, the mixture significantly inhibited TCDD-induced CYP1A activity (detected only by EROD assays) in human hepatoma cells.
Databáze: OpenAIRE
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