Relationships between sperm DNA fragmentation, sperm apoptotic markers and serum levels of CB-153 and p,p'-DDE in European and Inuit populations

Autor: Gunnar Toft, Lars Rylander, Marcello Spanò, D. Bizzaro, H. S. Pedersen, Gian Carlo Manicardi, Bonde Jp, Anna Rignell-Hydbom, Aleksander Giwercman, Jan K Ludwicki, Eva Cecilie Bonefeld-Jørgensen, Bo Jönsson, M Cecati, A Stronati, M Bordicchia, Vladimir Lesovoy, L. Ferrante, D. Sakkas
Rok vydání: 2006
Předmět:
Adult
Male
Embryology
Dichlorodiphenyl Dichloroethylene
Population
Greenland
CHROMATIN-STRUCTURE ASSAY
EJACULATED HUMAN SPERMATOZOA
MALE REPRODUCTIVE FUNCTION
POLYCHLORINATED-BIPHENYLS
STRAND BREAKS
PCB METABOLITES
ENVIRONMENTAL EXPOSURE
POSSIBLE INVOLVEMENT
MALE-INFERTILITY
SEMEN QUALITY
bcl-X Protein
Apoptosis
DNA Fragmentation
Biology
White People
Andrology
Semen quality
Endocrinology
Semen
TheoryofComputation_ANALYSISOFALGORITHMSANDPROBLEMCOMPLEXITY
In Situ Nick-End Labeling
Humans
fas Receptor
education
Sweden
education.field_of_study
TUNEL assay
Hormone activity
Sperm Count
Obstetrics and Gynecology
Cell Biology
Environmental exposure
Environmental Exposure
Flow Cytometry
Sperm
Polychlorinated Biphenyls
Spermatozoa
Reproductive Medicine
Inuit
Immunology
Linear Models
DNA fragmentation
Environmental Pollutants
Poland
Ukraine
Spermatogenesis
Biomarkers
Zdroj: Stronati, A, Manicardi, G C, Cecati, M, Bordicchia, M, Ferrante, L, Spanò, M, Toft, G, Bonde, J P, Jönsson, B A G, Rignell-Hydbom, A, Rylander, L, Giwercman, A, Pedersen, H S, Bonefeld-Jørgensen, E C, Ludwicki, J K, Lesovoy, V, Sakkas, D & Bizzaro, D 2006, ' Relationships between sperm DNA fragmentation, sperm apoptotic markers and serum levels of CB-153 and p,p'-DDE in European and Inuit populations ', Reproduction, vol. 132, no. 6, pp. 949-958 . https://doi.org/10.1530/rep.1.01034
ISSN: 1470-1626
DOI: 10.1530/rep.1.01034
Popis: Persistent organochlorine pollutants (POPs) are suspected to interfere with hormone activity and the normal homeostasis of spermatogenesis. We investigated the relationships between sperm DNA fragmentation, apoptotic markers identified on ejaculated spermatozoa and POP levels in the blood of 652 adult males (200 Inuits from Greenland, 166 Swedish, 134 Polish and 152 Ukrainian). Serum levels of 2, 2', 4, 4', 5, 5'-hexachlorobiphenyl (CB-153), as a proxy of the total POP burden, and of 1,1-dichloro-2,2-bis(p-chlorophenyl)-ethylene (p,p'-DDE), as a proxy of the total DDT exposure were determined. Sperm DNA fragmentation was measured by using the TUNEL assay, whereas immunofluorescence methods were utilized for detecting pro-apoptotic (Fas) and anti-apoptotic (Bcl-xL) markers. Both TUNEL assay and apoptotic markers were statistically differed across the four populations. No correlation between neither sperm DNA fragmentation nor apoptotic sperm parameters and the large variations in POPs exposure was observed for the separate study groups. However, considering the European populations taken together, we showed that both %TUNEL positivity and Bcl-xL were related to CB-153 serum levels, whereas our study failed to demonstrate any relations between DDE and %TUNEL positivity and apoptotic sperm biomarkers (Fas and Bcl-xL) in any region or overall regions. These results suggest that CB-153 and related chemicals might alter sperm DNA integrity and Bcl-xL levels in European adult males, but not in the highly exposed Inuit men. Additional issues (genetic background, lifestyle habits and characterization of total xeno-hormonal activities) need to be investigated in order to fully assess the population variations observed.
Databáze: OpenAIRE
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