Two genomic regions encoding exopolysaccharide production systems have complementary functions in B. cereus multicellularity and host interaction
| Autor: | Diego Romero, Antonio de Vicente, Yohanna Arboleda-Estudillo, Zahira Heredia-Ponce, Elena González-Muñoz, Ana Álvarez-Mena, Juan Antonio Guadix, Joaquín Caro-Astorga, Jesús Hierrezuelo |
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| Přispěvatelé: | [Caro-Astorga,J, Álvarez-Mena,A, Hierrezuelo,J, Heredia-Ponce,Z, de Vicente,A, Romero,D] Instituto de Hortofruticultura Subtropical y Mediterránea 'La Mayora' –Departamento de Microbiología, Universidad de Málaga, Málaga, Spain. [Guadix,JA] Departamento de Biología Animal, Facultad de Ciencias, Universidad de Málaga - IBIMA, Málaga, Spain. [Guadix,JA] Centro Andaluz de Nanomedicina y Biotecnología (BIONAND), Junta de Andalucía, Universidad de Málaga, Campanillas (Málaga), Spain. [Arboleda-Estudillo,Y, González-Munoz,E] LARCEL, Andalusian Laboratory of Cell Reprogramming, Andalusian Center for Nanomedicine and Biotechnology-BIONAND, Málaga, Spain., This work was supported by grants [AGL2012-31968 and AGL2016-78662-R] from the Ministerio de Economía y Competitividad, the Spanish Government and the European Research Council Starting Grant under Grant [BacBio 637971]. |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Organisms::Bacteria::Endospore-Forming Bacteria::Gram-Positive Endospore-Forming Bacteria::Gram-Positive Endospore-Forming Rods::Bacillaceae::Bacillus::Bacillus cereus [Medical Subject Headings] Cell Anatomy::Cells [Medical Subject Headings] lcsh:Medicine Extracellular matrix Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings] Microbial ecology Gene expression lcsh:Science Zebrafish education.field_of_study Multidisciplinary biology Virulence Extracellular Polymeric Substance Matrix Polysaccharides Bacterial Genomics Chemicals and Drugs::Carbohydrates::Polysaccharides [Medical Subject Headings] Cell biology Chemicals and Drugs::Carbohydrates::Polysaccharides::Polysaccharides Bacterial [Medical Subject Headings] medicine.anatomical_structure Cereus Comunicación celular Disciplines and Occupations::Natural Science Disciplines::Biological Science Disciplines::Biology::Genetics::Genomics [Medical Subject Headings] Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression Regulation::Gene Expression Regulation Bacterial [Medical Subject Headings] Genómica Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Bacterial Proteins [Medical Subject Headings] 030106 microbiology Population Bacterial adhesion Pez cebra Adhesión bacteriana Cell communication Article 03 medical and health sciences Bacillus cereus Bacterial Proteins medicine education Virulencia lcsh:R Biofilm Gene Expression Regulation Bacterial biology.organism_classification 030104 developmental biology Biofilms lcsh:Q Function (biology) Expresión génica |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-15 (2020) |
| ISSN: | 2045-2322 |
| Popis: | Bacterial physiology and adaptation are influenced by the exopolysaccharides (EPS) they produce. These polymers are indispensable for the assembly of the biofilm extracellular matrix in multiple bacterial species. In a previous study, we described the profound gene expression changes leading to biofilm assembly in B. cereus ATCC14579 (CECT148). We found that a genomic region putatively dedicated to the synthesis of a capsular polysaccharide (eps2) was overexpressed in a biofilm cell population compared to in a planktonic population, while we detected no change in the transcript abundance from another genomic region (eps1) also likely to be involved in polysaccharide production. Preliminary biofilm assays suggested a mild role for the products of the eps2 region in biofilm formation and no function for the products of the eps1 region. The aim of this work was to better define the roles of these two regions in B. cereus multicellularity. We demonstrate that the eps2 region is indeed involved in bacterial adhesion to surfaces, cell-to-cell interaction, cellular aggregation and biofilm formation, while the eps1 region appears to be involved in a kind of social bacterial motility. Consistent with these results, we further demonstrate using bacterial-host cell interaction experiments that the eps2 region is more relevant to the adhesion to human epithelial cells and the zebrafish intestine, suggesting that this region encodes a bacterial factor that may potentiate gut colonization and enhance pathogenicity against humans. |
| Databáze: | OpenAIRE |
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