Bax-induced cell death of Arabidopsis is meditated through reactive oxygen-dependent and -independent processes
| Autor: | Jeong Dong Bahk, Dae-Jin Yun, Jae Cheol Jeong, Chae Oh Lim, Yoon Duck Koo, Sang-Soo Kwak, Doh Hoon Kim, Jaesung Nam, Maki Kawai-Yamada, Sang Yeol Lee, Dongwon Baek, Ji-Young Lee, Woo Sik Chung, Hirofumi Uchimiya, Jong Chan Hong |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Programmed cell death
Time Factors Recombinant Fusion Proteins Population Green Fluorescent Proteins Arabidopsis Gene Expression Apoptosis Plant Science DNA Fragmentation Mitochondrion Biology Models Biological Dexamethasone Green fluorescent protein Mice Transformation Genetic Genetics Arabidopsis thaliana Animals education Cell Size bcl-2-Associated X Protein education.field_of_study BAX inhibitor 1 Arabidopsis Proteins Protoplasts Membrane Proteins General Medicine biology.organism_classification Plants Genetically Modified Molecular biology Cell biology Mitochondria Luminescent Proteins Microscopy Fluorescence Proto-Oncogene Proteins c-bcl-2 Vacuoles Reactive Oxygen Species Agronomy and Crop Science Signal Transduction |
| Zdroj: | Plant molecular biology. 56(1) |
| ISSN: | 0167-4412 |
| Popis: | An Arabidopsis protoplast system was developed for dissecting plant cell death in individual cells. Bax, a mammalian pro-apoptotic member of the Bcl-2 family, induces apoptotic-like cell death in Arabidopsis. Bax accumulation in Arabidopsis mesophyll protoplasts expressing murine Bax cDNA from a glucocorticoid-inducible promoter results in cytological characteristics of apoptosis, namely DNA fragmentation, increased vacuolation, and loss of plasma membrane integrity. In vivo targeting analysis monitored using jellyfish green fluorescent protein (GFP) reporter indicated full-length Bax was localized to the mitochondria, as it does in animal cells. Deletion of the carboxyl-terminal transmembrane domain of Bax completely abolished targeting to mitochondria. Bax expression was followed by reactive oxygen species (ROS) accumulation. Treatment of protoplasts with the antioxidant N -acetyl- -cysteine (NAC) during induction of Bax expression strongly suppressed Bax-mediated ROS production and the cell death phenotype. However, some population of the ROS depleted cells still induced cell death, indicating that there is a process that Bax-mediated plant cell death is independent of ROS accumulation. Accordingly, suppression of Bax-mediated plant cell death also takes place in two different processes. Over-expression of a key redox-regulator, Arabidopsis nucleoside diphosphate kinase 2 (AtNDPK2) down-regulated ROS accumulation and suppressed Bax-mediated cell death and transient expression of Arabidopsis Bax inhibitor-1 (AtBI-1) substantially suppressed Bax-induced cell death without altering cellular ROS level. Taken together, our results collectively suggest that the Bax-mediated cell death and its suppression in plants is mediated by ROS-dependent and -independent processes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink