Oral Soft Tissue Regeneration Using Nano Controlled System Inducing Sequential Release of Trichloroacetic Acid and Epidermal Growth Factor
| Autor: | Suk-Won Lee, Kwang Man Park, Heithem Ben Amara, Sang Cheon Lee, Richard Leesungbok, Ki-Tae Koo, Hong Jae Lee, Kwantae Noh |
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| Rok vydání: | 2020 |
| Předmět: |
0206 medical engineering
Gingiva Biomedical Engineering Gene Expression Medicine (miscellaneous) 02 engineering and technology 03 medical and health sciences chemistry.chemical_compound Dogs Epidermal growth factor Nano Animals Humans Regeneration Trichloroacetic Acid Trichloroacetic acid Cell Proliferation 030304 developmental biology Chitosan Drug Carriers 0303 health sciences Epidermal Growth Factor Palate Chemistry Regeneration (biology) Significant difference Soft tissue Fibroblasts 020601 biomedical engineering Controlled release Drug Liberation Biophysics Fibroblast Growth Factor 1 Nanoparticles Original Article Hydrophobic and Hydrophilic Interactions Cell signaling pathways |
| Zdroj: | Tissue Eng Regen Med |
| ISSN: | 2212-5469 1738-2696 |
| DOI: | 10.1007/s13770-019-00232-9 |
| Popis: | BACKGROUND: The effect of nano controlled sequential release of trichloroacetic acid (TCA) and epidermal growth factor (EGF) on the oral soft tissue regeneration was determined. METHODS: Hydrophobically modified glycol chitosan (HGC) nano controlled system was developed for the sequential release of TCA and EGF, and the release pattern was identified. The HGC-based nano controlled release system was injected into the critical-sized defects created in beagles’ palatal soft tissues. The palatal impression and its scanned body was obtained on various time points post-injection, and the volumetric amount of soft tissue regeneration was compared among the three groups: CON (natural regeneration control group), EXP1 (TCA-loaded nano controlled release system group), EXP2 (TCA and EGF individually loaded nano controlled release system). DNA microarray analysis was performed and various soft tissue regeneration parameters in histopathological specimens were measured. RESULTS: TCA release was highest at Day 1 whereas EGF release was highest at Day 2 and remained high until Day 3. In the volumetric measurements of impression body scans, no significant difference in soft tissue regeneration between the three groups was shown in two-way ANOVA. However, in the one-way ANOVA at Day 14, EXP2 showed a significant increase in soft tissue regeneration compared to CON. High correlation was determined between the histopathological results of each group. DNA microarray showed up-regulation of various genes and related cell signaling pathways in EXP2 compared to CON. CONCLUSION: HGC-based nano controlled release system for sequential release of TCA and EGF can promote regeneration of oral soft tissue defects. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13770-019-00232-9) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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