Pathophysiological significance of a reaction in mouse gastrointestinal tract associated with delayed-type hypersensitivity
| Autor: | Hui Pan, Ping Lin, Wan-Gui Yu, Lin Mei, En-Cong Gong, Lan Xiao |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Male
Leukocyte migration medicine.medical_treatment H&E stain Drug Hypersensitivity Mice immune system diseases medicine Dinitrochlorobenzene Animals Hypersensitivity Delayed Gastrointestinal tract Mice Inbred BALB C business.industry Gastroenterology General Medicine Enema medicine.disease Ulcerative colitis Small intestine Pathophysiology Hematochezia Gastrointestinal Tract medicine.anatomical_structure Basic Research Immunology Irritants medicine.symptom business |
| Zdroj: | World journal of gastroenterology. 10(15) |
| ISSN: | 1007-9327 |
| Popis: | AIM: To explore the pathophysiological significance of delayed type hypersensitivity (DTH) reaction in mouse gastrointestinal tract induced by an allergen 2,4-dinitrochlorobenzene (DNCB). METHODS: BALB/c mice were randomly divided into control and DTH1-6 groups. After sensitized by DNCB smeared on the abdominal skin, the mice were challenged with DNCB by gavage or enema. The weight, stool viscosity and hematochezia were observed and accumulated as disease active index (DAI) score; the gastrointestinal motility was represented by active charcoal propulsion rate; the colon pathological score was achieved by macropathology and HE staining of section prepared for microscopy; and the leukocyte migration inhibitory factor (LMIF) activity was determined by indirect capillary assay of the absorbance (A) of migrated leukocytes. RESULTS: Active charcoal propulsion rates of small intestine in the DNCB gavages groups were significantly higher than that in the control group (P < 0.01). The DAI scores and pathological score in DNCB enema groups were also higher than that in the control group ( P < 0.05), and there were significant rises in LMIF activity in DNCB enema groups as compared with control groups (P < 0.01). CONCLUSION: Mouse gastrointestinal DTH reaction could be induced by DNCB, which might facilitate the mechanism underlying the ulcerative colitis. |
| Databáze: | OpenAIRE |
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