Polymeric microparticles for sustained and local delivery of antiCD40 and antiCTLA-4 in immunotherapy of cancer
| Autor: | Rahimian, Sima, Fransen, Marieke F., Kleinovink, Jan Willem, Amidi, Maryam, Ossendorp, Ferry, Hennink, Wim E., Sub Drug delivery, Dep Farmaceutische wetenschappen, UIPS - Utrecht Institute for Pharmaceutical Sciences, Pharmaceutics |
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| Přispěvatelé: | Sub Drug delivery, Dep Farmaceutische wetenschappen, UIPS - Utrecht Institute for Pharmaceutical Sciences, Pharmaceutics |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Local delivery
Materials science Cell Survival medicine.medical_treatment CTLA4-blocking antibody Biophysics Capsules Cancer immunotherapy Bioengineering Pharmacology AntiCD40 Incomplete Freund's adjuvant (IFA) Antibodies PLHMGA Diffusion Biomaterials Mice In vivo Cell Line Tumor medicine Animals Immunologic Factors CTLA-4 Antigen CD40 Antigens CD40 biology Cancer Immunotherapy Neoplasms Experimental medicine.disease Immunomodulatory antibody In vitro Mice Inbred C57BL Treatment Outcome Mechanics of Materials Delayed-Action Preparations biology.protein Ceramics and Composites Antibody Adjuvant Sustained release |
| Zdroj: | Biomaterials, 61, 33. Elsevier Ltd Biomaterials, 61, 33-40 |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/j.biomaterials.2015.04.043 |
| Popis: | This study investigated the feasibility of the use of polymeric microparticles for sustained and local delivery of immunomodulatory antibodies in immunotherapy of cancer. Local delivery of potent immunomodulatory antibodies avoids unwanted systemic side effects while retaining their anti-tumor effects. Microparticles based on poly(lactic-co-hydroxymethyl-glycolic acid) (pLHMGA) and loaded with two distinct types of immunomodulatory antibodies (CTLA-4 antibody blocking inhibitory receptors on T cells or CD40 agonistic antibody stimulating dendritic cells) were prepared by double emulsion solvent evaporation technique. The obtained particles had a diameter of 12-15 μm to avoid engulfment by phagocytes and were slightly porous as shown by SEM analysis. The loading efficiency of the antibodies in the microparticles was >85%. The in vitro release profile of antiCD40 and antiCTLA-4 from microparticles showed a burst release of about 20% followed by a sustained release of the content up to 80% of the loading in around 30 days. The therapeutic efficacy of the microparticulate formulations was studied in colon carcinoma tumor model (MC-38). Mice bearing subcutaneous MC-38 tumors were treated with the same dose of immunomodulatory antibodies formulated either in incomplete Freund's adjuvant (IFA) or in microparticles. The antibody-loaded microparticles showed comparable therapeutic efficacy to the IFA formulation with no local adverse effects. The biodegradable microparticles were fully resorbed in vivo and no remnants of inflammatory depots as observed with IFA were present in the cured mice. Moreover the microparticles exhibited lower antibody serum levels in comparison with IFA formulations which lowers the probability of systemic adverse effects. In conclusion, pLHMGA microparticles are excellent delivery systems in providing long-lasting and non-toxic antibody therapy for immunotherapy of cancer. |
| Databáze: | OpenAIRE |
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