In vitro evaluation of the efficacy of peach stones as mycotoxin binders
| Autor: | D Mirjana Stojanovic, J Milan Adamovic, S Aleksandra Bocarov-Stancic, R Vladimir Pantic, R Zorica Lopicic, V Jelena Milojkovic |
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| Rok vydání: | 2013 |
| Předmět: |
Ochratoxin A
Fusarium Aflatoxin Raw material 01 natural sciences Diacetoxyscirpenol chemistry.chemical_compound 0404 agricultural biotechnology Vomitoxin mycotoxins Food science lcsh:Science (General) Mycotoxin Zearalenone acid modification General Environmental Science 2. Zero hunger peach stones biology 010401 analytical chemistry in vitro 04 agricultural and veterinary sciences biology.organism_classification 040401 food science 0104 chemical sciences chemistry adsorption General Earth and Planetary Sciences lcsh:Q1-390 |
| Zdroj: | Zbornik Matice Srpske za Prirodne Nauke, Vol 2013, Iss 124, Pp 287-296 (2013) |
| ISSN: | 2406-0828 0352-4906 |
| Popis: | The paper describes in vitro model for the evaluation of ability of peach shell (unmodified and modified), prepared at the Institute for Technology of Nuclear and Other Mineral Raw Materials, Belgrade, to adsorb different mycotoxins. Peach stones were obtained from “Vino Župa” Company from Aleksandrovac, where they have been disposed of as by-products from their Juice Factory. After proper preparation, two sorts of peach shell particles were used: one as unmodified peach shell particles (PS) and another one obtained by acid modification, denoted as MPS. Adsorption of six mycotoxins: aflatoxin B1 (AFL), ochratoxin A (OTA), deoxynivalenol (DON), zearalenone (ZON), diacetoxyscirpenol (DAS) and T-2 toxin by PS and MPS was tested in vitro. Crude extracts of mycotoxins, produced at the Department of Microbiology of Bio-ecological Center, Zrenjanin, were used for adsorption experiments. The ability of binding mycotoxins was evaluated in the electrolyte 0.1 M K2HPO4, which pH value was adjusted to 3.0 and 7.0, respectively. Mass ratio of individual mycotoxin and peach shell samples was 1:5000. The experimental mixtures were incubated for 1 hour on a rotary shaker (185 rpm) at room temperature (22-25oC). After incubation, the extractions of non-adsorbed mycotoxins from the filtrates were performed with organic solvents, and their quantification was done by thin-layer chromatography (TLC). [Projekat Ministarstva nauke Republike Srbije, br. TR 31003: Development of technologies and products based on mineral raw materials and waste biomass for protection of natural resources for safe food production i br. TR 31023: Reduction of toxigenic Fusarium species and their mycotoxins in production of safe cereal-based foods] |
| Databáze: | OpenAIRE |
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