TNF-α Induces Phosphorylation of p47phox in Human Neutrophils: Partial Phosphorylation of p47phox Is a Common Event of Priming of Human Neutrophils by TNF-α and Granulocyte-Macrophage Colony-Stimulating Factor
| Autor: | Marie-Anne Gougerot-Pocidalo, Pham My-Chan Dang, Cedric Dewas, Jamel El-Benna |
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| Rok vydání: | 2003 |
| Předmět: |
Phosphopeptides
inorganic chemicals Neutrophils Immunology Peptide Mapping Neutrophil Activation Receptors Tumor Necrosis Factor Proinflammatory cytokine Adjuvants Immunologic Antigens CD Humans Immunology and Allergy Phosphorylation Tyrosine Protein kinase A Receptor Respiratory Burst NADPH oxidase biology Tumor Necrosis Factor-alpha Granulocyte-Macrophage Colony-Stimulating Factor NADPH Oxidases hemic and immune systems Phosphoproteins Molecular biology Enzyme Activation N-Formylmethionine Leucyl-Phenylalanine Kinetics Receptors Tumor Necrosis Factor Type I biology.protein Tumor necrosis factor alpha Tyrosine kinase Interleukin-1 Signal Transduction |
| Zdroj: | The Journal of Immunology. 171:4392-4398 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.171.8.4392 |
| Popis: | Phosphorylation of p47phox is a key event in NADPH oxidase activation. We examined the ability of proinflammatory cytokines such as TNFα, IL-1, and G-CSF to induce this process compared with GM-CSF. Only TNF-α and GM-CSF induced a clear p47phox phosphorylation. This phosphorylation was time dependent and reached its maximum at 20 min. Two-dimensional phosphopeptide mapping of p47phox phosphorylated in neutrophils primed with TNF-α revealed partial phosphorylation of p47phox on the same peptide as for GM-CSF. Neutrophil incubation with TNF-α and subsequent addition of the chemotactic peptide fMLP resulted in more intense phosphorylation of p47phox sites than with each reagent alone. A neutralizing Ab against the p55 TNF receptor, contrary to a neutralizing Ab against the p75 TNF receptor, inhibited TNF-α-induced p47phox phosphorylation. Neutrophil treatment with both TNF-α and GM-CSF resulted in more intense phosphorylation of the same p47phox peptide observed with each cytokine alone, suggesting that they engaged pathways converging on common serines. This additive effect was also obtained on the priming of NADPH oxidase activity. The use of protein kinase inhibitors pointed to the involvement of a protein tyrosine kinase, but not protein kinase C. These findings show that TNF-α, via its p55 receptor, induces a protein tyrosine kinase-dependent selective phosphorylation of p47phox on specific serines. The ability of TNF-α and GM-CSF, two different cytokines with two different receptors to induce this specific p47phox phosphorylation, suggests that this event could be a common element of the priming of neutrophils by TNF-α and GM-CSF. |
| Databáze: | OpenAIRE |
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