Metabolism of Papaverine III. Effect of Phenobarbital, 3-Methylcholanthrene and SKF 525-A Pre-treatmentin vivoandin vitro
| Autor: | Frans M. Belpaire, Bogaert Mg |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Male
Oxidoreductases O-Demethylating Health Toxicology and Mutagenesis Biliary Elimination Pharmacology Toxicology Biochemistry chemistry.chemical_compound Cytochrome P-450 Enzyme System In vivo Papaverine medicine Animals Bile Proadifen Organ Size General Medicine Metabolism In vitro Rats Skf 525 a Liver chemistry Phenobarbital Injections Intravenous Methylcholanthrene Chromatography Thin Layer Injections Intraperitoneal Aminopyrine N-Demethylase medicine.drug |
| Zdroj: | Xenobiotica. 5:431-438 |
| ISSN: | 1366-5928 0049-8254 |
| Popis: | 1. The metabolism of papaverine was studied in vitro using the 9000 g fraction of rat liver. Apparent Km and Vmax values were 1.6 X 10-5 M and 34 nmol/g/min. Three monodemethylated metabolites were found by t.l.c. 2. Pre-treatment of rats with phenobarbital enhanced the activity of papaverine O-demethylase, whereas pre-treatment with 3-methylcholanthrene did not. Pre-treatment with SKF 525-A, or addition of AKF 525-A invitro markedly inhibited the enzyme. 3. Pre-treatment of the rats with phenobarbital enhanced the biliary elimination rate of papaverine metabolites, whereas SKF 525-A pre-treatment slowed this elimination. |
| Databáze: | OpenAIRE |
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