A Smartphone-based Diffusometric Immunoassay for Detecting C-Reactive Protein

Autor:	Yu Jui Fan, Chih Shen Chuang, Hong-Ren Jiang, Yi Fan Fang, Chih Zong Deng, Horn-Jiunn Sheen, Pao Wei Tseng
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	Diffusion lcsh:Medicine 02 engineering and technology Biosensing Techniques Immunologic Tests 01 natural sciences Biochemical assays Article Fluorescence medicine lcsh:Science Immunoassay Multidisciplinary Chromatography medicine.diagnostic_test Chemistry 010401 analytical chemistry lcsh:R Health care 021001 nanoscience & nanotechnology 0104 chemical sciences C-Reactive Protein Spectrometry Fluorescence Nanoparticles lcsh:Q Smartphone 0210 nano-technology
Zdroj:	Scientific Reports, Vol 9, Iss 1, Pp 1-7 (2019) Scientific Reports
ISSN:	2045-2322
Popis:	In this study, we developed a portable smartphone-based diffusometry for analyzing the C-reactive protein (CRP) concentration. An optimized fluorescence microscopic add-on system for a smartphone was used to image the 300 nm fluorescent beads. Sequential nanobead images were recorded for a period and the image data were used for fluorescence correlation spectrometric (FCS) analysis. Through the analysis, the nanobeads’ diffusion coefficient was obtained. Further, the diffusion coefficients of the anti-CRP-coated nanobeads, which were suspended in the samples with various CRP concentrations, were estimated using smartphone-based diffusometry. After 10 min of reaction, the anti-CRP-coated nanobeads in a higher CRP concentration solution led to a lower diffusion coefficient. Based on the experiments, a linear sensing range of 1~8 µg/mL was found.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::25c35f9f8f868d20d132f154833c9a79 http://link.springer.com/article/10.1038/s41598-019-52285-4 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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