A Smartphone-based Diffusometric Immunoassay for Detecting C-Reactive Protein
| Autor: | Yu Jui Fan, Chih Shen Chuang, Hong-Ren Jiang, Yi Fan Fang, Chih Zong Deng, Horn-Jiunn Sheen, Pao Wei Tseng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Diffusion
lcsh:Medicine 02 engineering and technology Biosensing Techniques Immunologic Tests 01 natural sciences Biochemical assays Article Fluorescence medicine lcsh:Science Immunoassay Multidisciplinary Chromatography medicine.diagnostic_test Chemistry 010401 analytical chemistry lcsh:R Health care 021001 nanoscience & nanotechnology 0104 chemical sciences C-Reactive Protein Spectrometry Fluorescence Nanoparticles lcsh:Q Smartphone 0210 nano-technology |
| Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-7 (2019) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-019-52285-4 |
| Popis: | In this study, we developed a portable smartphone-based diffusometry for analyzing the C-reactive protein (CRP) concentration. An optimized fluorescence microscopic add-on system for a smartphone was used to image the 300 nm fluorescent beads. Sequential nanobead images were recorded for a period and the image data were used for fluorescence correlation spectrometric (FCS) analysis. Through the analysis, the nanobeads’ diffusion coefficient was obtained. Further, the diffusion coefficients of the anti-CRP-coated nanobeads, which were suspended in the samples with various CRP concentrations, were estimated using smartphone-based diffusometry. After 10 min of reaction, the anti-CRP-coated nanobeads in a higher CRP concentration solution led to a lower diffusion coefficient. Based on the experiments, a linear sensing range of 1~8 µg/mL was found. |
| Databáze: | OpenAIRE |
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