Ten–Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis
| Autor: | Kai F. Hoettges, Patrizia Camelliti, Rula G. Abdallat, Christopher H. Fry, Erin A. Henslee, Daan R. van der Veen, A Waheed, Andrew D. Beale, Michael P. Hughes, Rita I. Jabr, Ruth M. Torcal Serrano, Fatima H. Labeed |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Blood Platelets Electrophoresis Materials science Erythrocytes Time Factors Cells Dielectrophoresis lcsh:Medicine Cell analysis Membrane trafficking 01 natural sciences Capacitance Article 03 medical and health sciences Membrane biophysics Jurkat Cells Mice Animals Humans Membrane conductance Circadian rhythms lcsh:Science membrane Electrodes Ultradian rhythm Reproducibility Electrorotation DEP Multidisciplinary electrokinetics Arvicolinae lcsh:R 010401 analytical chemistry Cell Membrane Osmolar Concentration Electric Conductivity Ultradian Rhythm Limiting 0104 chemical sciences Electrophysiological Phenomena Electrophysiology 030104 developmental biology Electrode geometry lcsh:Q K562 Cells Biomedical engineering |
| Zdroj: | Scientific Reports Hoettges, K F, Henslee, E, Torcal Serrano, R M, Jabr, R, Abdallat, R, Beale, A, Waheed, A, Camelliti, P, Fry, C H, van der Veen, D, Labeed, F H & Hughes, M 2019, ' Ten–Second Electrophysiology : Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis ', Scientific Reports, vol. 9, 19153 (2019) . https://doi.org/10.1038/s41598-019-55579-9 Scientific Reports, Vol 9, Iss 1, Pp 1-13 (2019) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-019-55579-9 |
| Popis: | Electrical correlates of the physiological state of a cell, such as membrane conductance and capacitance, as well as cytoplasm conductivity, contain vital information about cellular function, ion transport across the membrane, and propagation of electrical signals. They are, however, difficult to measure; gold-standard techniques are typically unable to measure more than a few cells per day, making widespread adoption difficult and limiting statistical reproducibility. We have developed a dielectrophoretic platform using a disposable 3D electrode geometry that accurately (r2 > 0.99) measures mean electrical properties of populations of ~20,000 cells, by taking parallel ensemble measurements of cells at 20 frequencies up to 45 MHz, in (typically) ten seconds. This allows acquisition of ultra-high-resolution (100-point) DEP spectra in under two minutes. Data acquired from a wide range of cells – from platelets to large cardiac cells - benchmark well with patch-clamp-data. These advantages are collectively demonstrated in a longitudinal (same-animal) study of rapidly-changing phenomena such as ultradian (2–3 hour) rhythmicity in whole blood samples of the common vole (Microtus arvalis), taken from 10 µl tail-nick blood samples and avoiding sacrifice of the animal that is typically required in these studies. |
| Databáze: | OpenAIRE |
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