Microarray studies reveal a ‘differential response’ to moderate or severe heat shock of the HrcA- and HspR-dependent systems in Corynebacterium glutamicum
| Autor: | Carlos Barreiro, Diana Nakunst, Jörn Kalinowski, Andrea T. Hüser, Héctor D. de Paz, Juan F. Martín |
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| Rok vydání: | 2009 |
| Předmět: |
Regulation of gene expression
biology Transcription Genetic Repressor Gene Expression Regulation Bacterial Molecular biology Microbiology Corynebacterium glutamicum Retraction DNA-Binding Proteins Repressor Proteins Bacterial Proteins Chaperone (protein) Heat shock protein Cell and Molecular Biology of Microbes biology.protein Northern blot Heat shock Gene Heat-Shock Proteins Heat-Shock Response Oligonucleotide Array Sequence Analysis |
| Zdroj: | Microbiology |
| ISSN: | 1465-2080 1350-0872 |
| DOI: | 10.1099/mic.0.019299-0 |
| Popis: | Genome-wide transcription profile analysis of the heat-shocked wild-type strain under moderate (40 °C) and severe heat stress (50 °C) revealed that a large number of genes are differentially expressed after heat shock. Of these, 358 genes were upregulated and 420 were downregulated in response to moderate heat shock (40 °C) inCorynebacterium glutamicum. Our results confirmed the HrcA/controlling inverted repeat of chaperone expression (CIRCE)-dependent and HspR/HspR-associated inverted repeat (HAIR)-dependent upregulation of chaperones following heat shock. Other genes, including clusters of orthologous groups (COG) related to macromolecule biosynthesis and several transcriptional regulators (COG class K), were upregulated, explaining the large number of genes affected by heat shock. Mutants having deletions in thehrcAorhspRregulators were constructed, which allowed the complete identification of the genes controlled by those systems. The up- or downregulation of several genes observed in the microarray experiments was validated by Northern blot analyses and quantitative (real-time) reverse-transcription PCR. These analyses showed a heat-shock intensity-dependent response (‘differential response’) in the HspR/HAIR system, in contrast to the non-differential response shown by the HrcA/CIRCE-regulated genes. |
| Databáze: | OpenAIRE |
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