Distribution of enteric glia and GDNF during gut inflammation
| Autor: | M. Steinkamp, Christoph Hartmann, Georg B T von Boyen, Ulrike Spaniol, Nadine Schulte, Carolin Pflüger |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Colon animal diseases Inflammation Infectious Colitis Inflammatory bowel disease Enteric Nervous System Young Adult Crohn Disease Neurotrophic factors Glial Fibrillary Acidic Protein medicine Glial cell line-derived neurotrophic factor Humans Glial Cell Line-Derived Neurotrophic Factor lcsh:RC799-869 Colitis biology Glial fibrillary acidic protein business.industry urogenital system Gastroenterology General Medicine Middle Aged medicine.disease Ulcerative colitis digestive system diseases nervous system Immunology biology.protein lcsh:Diseases of the digestive system. Gastroenterology Colitis Ulcerative Female medicine.symptom business Neuroglia Biomarkers Research Article |
| Zdroj: | BMC Gastroenterology BMC Gastroenterology, Vol 11, Iss 1, p 3 (2011) |
| ISSN: | 1471-230X |
| Popis: | Background The enteric glia network may be involved in the pathogenesis of inflammatory bowel disease (IBD). Enteric glia cells (EGCs) are the major source of glial-derived neurotrophic factor (GDNF), which regulates apoptosis of enterocytes. The aim of the study was to determine the distribution of EGCs and GDNF during gut inflammation and to elucidate a possible diminished enteric glia network in IBD. Methods The expression of glial fibrillary acidic protein (GFAP) in colonic biopsies of patients with IBD, controls and patients with infectious colitis was detected by immunohistochemistry and Western blot. Tissue GDNF levels were measured by ELISA. Results The expression of GFAP and GDNF in the mucosal plexus is highly increased in the inflamed colon of patients with ulcerative colitis (UC) and infectious colitis. Although the GDNF and GFAP content are increased in Crohn's disease (CD), it is significantly less. Additionally the non-inflamed colon of CD patients showed a reduced GFAP and no GDNF expression compared to controls and the non-inflamed colon of UC patients. Conclusions GFAP and GDNF as signs of activated EGCs are increased in the inflamed mucosa of patients with UC and infectious colitis, which underline an unspecific role of EGC in the regulation of intestinal inflammation. The reduced GFAP and GDNF content in the colon of CD patients suggest a diminished EGC network in this disease. This might be a part of the pathophysiological puzzle of CD. |
| Databáze: | OpenAIRE |
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