A Novel Strategy for Creating Tissue-Engineered Biomimetic Blood Vessels Using 3D Bioprinting Technology
| Autor: | Hongyi Yao, Hu Yingying, Changyong Liu, Yuanyuan Xu, Boxun Liu, Shengli Mi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Scaffold Materials science Vascular smooth muscle 02 engineering and technology Matrix (biology) lcsh:Technology Article law.invention Extracellular matrix 03 medical and health sciences biomimetic modeling law dECM Adventitia medicine small-diameter blood vessels General Materials Science lcsh:Microscopy lcsh:QC120-168.85 3D bioprinting Decellularization lcsh:QH201-278.5 lcsh:T 021001 nanoscience & nanotechnology 030104 developmental biology medicine.anatomical_structure vascularized tissues lcsh:TA1-2040 cardiovascular system lcsh:Descriptive and experimental mechanics lcsh:Electrical engineering. Electronics. Nuclear engineering 0210 nano-technology lcsh:Engineering (General). Civil engineering (General) lcsh:TK1-9971 Blood vessel Biomedical engineering |
| Zdroj: | Materials Volume 11 Issue 9 Materials, Vol 11, Iss 9, p 1581 (2018) |
| ISSN: | 1996-1944 |
| DOI: | 10.3390/ma11091581 |
| Popis: | In this work, a novel strategy was developed to fabricate prevascularized cell-layer blood vessels in thick tissues and small-diameter blood vessel substitutes using three-dimensional (3D) bioprinting technology. These thick vascularized tissues were comprised of cells, a decellularized extracellular matrix (dECM), and a vasculature of multilevel sizes and multibranch architectures. Pluronic F127 (PF 127) was used as a sacrificial material for the formation of the vasculature through a multi-nozzle 3D bioprinting system. After printing, Pluronic F127 was removed to obtain multilevel hollow channels for the attachment of human umbilical vein endothelial cells (HUVECs). To reconstruct functional small-diameter blood vessel substitutes, a supporting scaffold (SE1700) with a double-layer circular structure was first bioprinted. Human aortic vascular smooth muscle cells (HA-VSMCs), HUVECs, and human dermal fibroblasts&ndash neonatal (HDF-n) were separately used to form the media, intima, and adventitia through perfusion into the corresponding location of the supporting scaffold. In particular, the dECM was used as the matrix of the small-diameter blood vessel substitutes. After culture in vitro for 48 h, fluorescent images revealed that cells maintained their viability and that the samples maintained structural integrity. In addition, we analyzed the mechanical properties of the printed scaffold and found that its elastic modulus approximated that of the natural aorta. These findings demonstrate the feasibility of fabricating different kinds of vessels to imitate the structure and function of the human vascular system using 3D bioprinting technology. |
| Databáze: | OpenAIRE |
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