Sugars from Sphacelia sorghi honeydew
| Autor: | G.R. Gray, R.L. Mower, Clinton E. Ballou |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Sucrose
Chromatography Gas Optical Rotation Chromatography Paper Stereochemistry Disaccharide Oligosaccharides Fructose Tritium Methylation Biochemistry Mass Spectrometry Analytical Chemistry chemistry.chemical_compound Hydrolysis medicine Moiety Organic chemistry Mannitol Organic Chemistry Periodate General Medicine Plants Paper chromatography Glucose chemistry Chromatography Gel Oxidation-Reduction medicine.drug |
| Zdroj: | Carbohydrate Research. 27:119-134 |
| ISSN: | 0008-6215 |
| DOI: | 10.1016/s0008-6215(00)82431-3 |
| Popis: | Several unusual oligosaccharides have been isolated from the honeydew of Sphacelia sorghi McRae. These include 1-O-β- D -fructofuranosyl- D -mannitol, 5-O-β- D -fructofuranosyl- D -arabinitol, 1,6-di-O-β- D -fructofuranosyl- D -mannitol, 1,5-di-O-β- D -fructofuranosyl- D -arabinitol, and 1-O-β- D -fructofuranosyl-6-O-[β- D -fructofuranosyl-(2→6)-β- D -fructofuranosyl]- D -mannitol. In addition to these oligosaccharides, D -glucose, D -fructose, D -arabinitol, D -mannitol, sucrose, and 6-O-β- D -fructofuranosyl- D -glucose were also found in the honeydew. The structures of the previously undescribed oligosaccharides were determined by periodate oxidation studies, their cleavage by β- D -fructofuranosidase, optical rotation measurements, and methylation analysis by combined gas-liquid chromatography-mass spectrometry. The position of linkage in the arabinitol-containing disaccharide was determined by incorporation of D -[1-3H]-arabinitol into a β- D -fructofuranosyl- D -arabinitol in vivo. The release of tritium-labeled formaldehyde during periodate oxidation of the product demonstrated that the β- D -fructofuranosyl moiety was linked to position 5 of the D -[1-3H]-arabinitol. |
| Databáze: | OpenAIRE |
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