Contribution of PsbS Function and Stomatal Conductance to Foliar Temperature in Higher Plants
| Autor: | Maciej Jerzy Bernacki, Stanislaw Karpinski, Damian Witoń, K. Ciszak, Milena Kulasek |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Stomatal conductance Thermal imaging Light Photosystem II Physiology Plant Science Photosynthesis Models Biological 01 natural sciences 03 medical and health sciences Botany Arabidopsis thaliana Special Focus Issue – Regular Papers Plant Proteins Transpiration Quenching (fluorescence) biology Chemistry PsbS Non-photochemical quenching Temperature Plant Transpiration Excess excitation energy dissipation Cell Biology General Medicine Plants biology.organism_classification Horticulture Light intensity 030104 developmental biology Organ Specificity Diuron Plant Stomata Linear Models Foliar temperature dynamics 010606 plant biology & botany |
| Zdroj: | Plant and Cell Physiology |
| ISSN: | 1471-9053 0032-0781 |
| Popis: | Natural capacity has evolved in higher plants to absorb and harness excessive light energy. In basic models, the majority of absorbed photon energy is radiated back as fluorescence and heat. For years the proton sensor protein PsbS was considered to play a critical role in non-photochemical quenching (NPQ) of light absorbed by PSII antennae and in its dissipation as heat. However, the significance of PsbS in regulating heat emission from a whole leaf has never been verified before by direct measurement of foliar temperature under changing light intensity. To test its validity, we here investigated the foliar temperature changes on increasing and decreasing light intensity conditions (foliar temperature dynamics) using a high resolution thermal camera and a powerful adjustable light-emitting diode (LED) light source. First, we showed that light-dependent foliar temperature dynamics is correlated with Chl content in leaves of various plant species. Secondly, we compared the foliar temperature dynamics in Arabidopsis thaliana wild type, the PsbS null mutant npq4-1 and a PsbS-overexpressing transgenic line under different transpiration conditions with or without a photosynthesis inhibitor. We found no direct correlations between the NPQ level and the foliar temperature dynamics. Rather, differences in foliar temperature dynamics are primarily affected by stomatal aperture, and rapid foliar temperature increase during irradiation depends on the water status of the leaf. We conclude that PsbS is not directly involved in regulation of foliar temperature dynamics during excessive light energy episodes. |
| Databáze: | OpenAIRE |
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