Surface plasmon resonance based indirect immunoassay for detection of 17β-estradiol
| Autor: | Vinod Joshi, Rakhee Gehlot, Urvasini Singh, Kavita Sharma, Sunita Kumbhat |
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| Rok vydání: | 2018 |
| Předmět: |
Clinical Biochemistry
Fluoroimmunoassay Pharmaceutical Science Metal Nanoparticles Biosensing Techniques 01 natural sciences Sensitivity and Specificity Antibodies Analytical Chemistry chemistry.chemical_compound Amide Drug Discovery medicine Surface plasmon resonance Fluorescent Antibody Technique Indirect Spectroscopy Detection limit Chromatography medicine.diagnostic_test Estradiol 010405 organic chemistry Chemistry 010401 analytical chemistry technology industry and agriculture Reproducibility of Results Self-assembled monolayer Surface Plasmon Resonance 0104 chemical sciences Molecular Weight Covalent bond Immunoassay Gold Biosensor Conjugate |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 163 |
| ISSN: | 1873-264X |
| Popis: | A surface plasmon resonance (SPR) based immunosensor is presented for highly sensitive and selective detection of 17β-estradiol by the indirect competitive inhibition immuno assay, employing anti-17 β-estradiol antibody as high molecular weight (HMW) interactant. Immobilization of estradiol-BSA conjugate onto the nano thin gold surface was accomplished by covalent amide linkage through self assembled monolayer. The proposed biosensor is simple to fabricate, reproducible and exhibit excellent sensitivity for estrogen (detection limit,1 pg mL−1) without any significant interference from structurally similar steroidal hormone, progesterone and non-steroidal compound bisphenol-A. The proposed surface displayed a high level of stability during repeated regeneration and immunoreaction cycles suitable for biosensor development. |
| Databáze: | OpenAIRE |
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