Hydrophilic Interaction Chromatography Using Methacrylate-Based Monolithic Capillary Column for the Separation of Polar Analytes
| Autor: | Paul D. Ferguson, Norman W. Smith, Mark R. Taylor, Zhengjin Jiang |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Chromatography
geography geography.geographical_feature_category Chemistry Capillary action Hydrophilic interaction chromatography Static Electricity Methacrylate Permeability Analytical Chemistry chemistry.chemical_compound Betaine Capillary Electrochromatography Copolymer Methacrylates Solid phase extraction Organic Chemicals Monolith Selectivity |
| Zdroj: | Analytical Chemistry. 79:1243-1250 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/ac061871f |
| Popis: | A porous zwitterionic monolith was prepared by thermal copolymerization of N,N-dimethyl-N-methacryloxyethyl-N-(3-sulfopropyl)ammonium betaine and ethylene dimethacrylate inside a 100-μm-i.d. capillary. The resulting monolith was evaluated as a hydrophilic liquid chromatography (HILIC) stationary phase. No evidence of swelling or shrinking of the monolith in different polarity solvents was observed. A typical HILIC mechanism was observed at higher organic solvent content (ACN% > 60%). The poly(SPE-co-EDMA) monolith showed very good selectivity for neutral, basic, and acidic polar analytes. For charged analytes, both hydrophilic interactions and electrostatic interactions contributed to their retention, which provide chromatographers more choice to optimize the separations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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