Enzymatic Transesterification of Coconut Oil Using Chitosan-Immobilized Lipase Produced by Fluidized-Bed System
| Autor: | Ana K.F. Carvalho, T. A. Costa-Silva, Wanderley Pereira de Oliveira, Suraia Said, Claudia R. F. Souza, H. F. de Castro |
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| Rok vydání: | 2017 |
| Předmět: |
food.ingredient
Chromatography biology Water activity Chemistry 020209 energy General Chemical Engineering Coconut oil Energy Engineering and Power Technology 02 engineering and technology Transesterification Chitosan chemistry.chemical_compound Fuel Technology food Fluidized bed 0202 electrical engineering electronic engineering information engineering biology.protein LIPASE Organic chemistry Epichlorohydrin Glutaraldehyde Lipase |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1520-5029 0887-0624 |
| DOI: | 10.1021/acs.energyfuels.7b02033 |
| Popis: | This work demonstrated the catalytic performance of Cercospora kikichii lipase immobilized onto chitosan acetate microparticles activated with different cross-linking agents and dried using a fluidized-bed system. The activating agent affected the lipase activity, and the highest immobilizing yield was achieved by the chitosan microparticles activated with 1.5% glutaraldehyde (93.7%) followed by activation with metaperiodate (78.9%) and epichlorohydrin (70.6%). The immobilized biocatalysts produced showed low moisture content and water activity, namely, 3.5% and 0.12, respectively; and high stability under storage conditions maintaining 77.7% of its initial activity after 6 months at 5 °C. The industrial applicability of the biocatalyst was assessed in the transesterification of coconut oil (Cocos nucifera oil) using ethanol as an acylant agent. The viscosity value for the coconut oil (29 mm2·s–1) sharply decreased to 3.2 mm2·s–1, upon the progress of transesterification reaction. This represents a final ... |
| Databáze: | OpenAIRE |
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