Three lymphoid-specific factors account for all junctional diversity characteristic of somatic assembly of T-cell receptor and immunoglobulin genes
| Autor: | François Rougeon, M Fanton d'Andon, Sacha Kallenbach, Noëlle Doyen |
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| Jazyk: | angličtina |
| Rok vydání: | 1992 |
| Předmět: |
Homeodomain Proteins
Recombination Genetic Multidisciplinary Genes Immunoglobulin Somatic cell Cellular differentiation T-cell receptor Receptors Antigen T-Cell Proteins Gene rearrangement 3T3 Cells Biology Gene Rearrangement T-Lymphocyte Transfection Molecular biology Junctional diversity Recombination-activating gene DNA-Binding Proteins Mice Terminal deoxynucleotidyl transferase RAG2 DNA Nucleotidylexotransferase Animals Gene Rearrangement B-Lymphocyte Research Article |
| Popis: | The somatic diversity immunoglobulin and T-cell receptor diversity is largely provided by the junctional variation created during site-specific rearrangement of separately encoded gene segments. Using a transient transfection assay, we demonstrate that the recombination activating genes Rag1 and Rag2 direct site-specific rearrangement on an artificial substrate in poorly differentiated as well as in differentiated nonlymphoid cell lines. In addition to a high frequency of precise recombination events, coding joints show deletions and more rarely P-nucleotide insertions, reminiscent of immunoglobulin and T-cell receptor junctions found in fetal tissues. N-region insertions, which are characteristic of adult junctional diversity, are obtained at high frequency upon transfection of a terminal deoxynucleotidyltransferase expression vector together with Rag1 and Rag2. These results show that only three lymphoid-specific factors are needed to generate all types of junctional diversity observed during lymphoid development. |
| Databáze: | OpenAIRE |
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