Rosehip Oil Promotes Excisional Wound Healing by Accelerating the Phenotypic Transition of Macrophages
| Autor: | Wenwen Jin, Longjiang Yu, Zaiwang Yang, Mingzhang Ao, Zhi-Yong Lei, Zhi-Jian Cao |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
Pathology medicine.medical_specialty H&E stain Pharmaceutical Science Scars Rosa 01 natural sciences Gas Chromatography-Mass Spectrometry Analytical Chemistry Mice Antigens CD Fibrosis Drug Discovery Poor wound healing medicine Animals Plant Oils Macrophage Rats Wistar Pharmacology Wound Healing integumentary system 010405 organic chemistry CD68 business.industry Macrophages Organic Chemistry Cell Differentiation medicine.disease 0104 chemical sciences 010404 medicinal & biomolecular chemistry Phenotype Complementary and alternative medicine Molecular Medicine medicine.symptom Wound healing business CD163 |
| Zdroj: | Planta Medica. 85:563-569 |
| ISSN: | 1439-0221 0032-0943 |
| DOI: | 10.1055/a-0725-8456 |
| Popis: | Poor wound healing is a major and global threat to public health. Efforts have been made to better understand the underlying mechanisms and develop effective remedies, though the advancements that have been made are still limited. As there are no effective and generally applicable therapies available for skin injuries and fibrosis, it is urgent to develop new drugs and therapies that facilitate wound healing and effectively improve scars. In this study, GC-MS analysis was performed to identify the chemical composition of rosehip oil. The excisional wound healing model and the carrageenan-induced paw edema method were respectively applied to evaluate the wound healing activity and anti-inflammatory activity of rosehip oil. Hematoxylin and eosin staining was used to assess the pathological changes of sections, and Sirius-red staining was performed to analyze the ratio of collagen I/III in wound tissues. Immunohistological staining for CD68, CCR7 (CD197), CD163, TGF-β1, and α-SMA was applied to determine the macrophage phenotypes transition (M1-to-M2) and demonstrate the scar-improving efficacy of rosehip oil on wound healing. Results showed that rosehip oil significantly promoted wound healing and effectively improved scars. This efficacy might be exerted by accelerating the macrophage phenotypes transition and inhibiting the process of epithelial-mesenchymal transition. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|