Identification of key lncRNAs in the carcinogenesis and progression of colon adenocarcinoma by co‐expression network analysis
| Autor: | Biyong Tan, Shi Jiang, Xingqiang Zhang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Oncology medicine.medical_specialty Microarray Carcinogenesis Adenocarcinoma Biology medicine.disease_cause Biochemistry Correlation 03 medical and health sciences 0302 clinical medicine Internal medicine Biomarkers Tumor medicine Humans Gene Regulatory Networks Molecular Biology Gene Survival analysis Gene Expression Profiling Cell Biology Lncrna expression Colon carcinogenesis Gene Expression Regulation Neoplastic 030104 developmental biology 030220 oncology & carcinogenesis Colonic Neoplasms Disease Progression RNA Long Noncoding Colon adenocarcinoma Neoplasm Recurrence Local |
| Zdroj: | Journal of Cellular Biochemistry. 120:6490-6501 |
| ISSN: | 1097-4644 0730-2312 |
| Popis: | Colon adenocarcinoma (COAD) is one of the most common cancers, and its carcinogenesis and progression is influenced by multiple long non-coding RNAs (lncRNA), especially through the miRNA sponge effect. In this study, more than 4000 lncRNAs were re-annotated from the microarray datasets through probe sequence mapping to obtain reliable lncRNA expression profiles. As a systems biology method for describing the correlation patterns among genes across microarray samples, weighted gene co-expression network analysis was conducted to identify lncRNA modules associated with the five stepwise stages from normal colonic samples to COAD (n = 94). In the most relevant module (R2 = -0.78, P = 4E-20), four hub lncRNAs were identified (CTD-2396E7.11, PCGF5, RP11-33O4.1, and RP11-164P12.5). Then, these four hub lncRNAs were validated using two other independent datasets including GSE20916 (n = 145) and GSE39582 (n = 552). The results indicated that all hub lncRNAs were significantly negatively correlated with the three-stage colonic carcinogenesis, as well as TNM stages in COAD (one-way analysis of variance P |
| Databáze: | OpenAIRE |
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