A single food bolus stimulates albumin synthesis in growing piglets
| Autor: | J.A.N. Meesterburrie, H.A.M. Voorbij, Dirk-Jan Reijngoud, K. de Meer, A. Okken, H. C. Smolders, W. Kulik, M.G.M. de Sain-van der Velden |
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| Přispěvatelé: | Faculteit Medische Wetenschappen/UMCG, Life Course Epidemiology (LCE) |
| Předmět: |
medicine.medical_specialty
Time Factors protein synthesis Swine growth Coefficient of variation stable isotopes liver OXIDATION Hydrolysate Eating Random Allocation Bolus (medicine) valine Valine Internal medicine medicine Animals Protein Precursors Infusions Intravenous Intubation Gastrointestinal Serum Albumin Carbon Isotopes Meal business.industry Gastroenterology Albumin HUMANS Fasting MASS-SPECTROMETRY STABLE-ISOTOPE Postprandial Period nutrition Endocrinology Postprandial Infant formula Isotope Labeling BODY PROTEIN-SYNTHESIS Models Animal Pediatrics Perinatology and Child Health Female Dietary Proteins MEAL business |
| Zdroj: | University of Groningen Journal of Pediatric Gastroenterology and Nutrition, 31(3), 251-257. Lippincott Williams and Wilkins |
| ISSN: | 0277-2116 |
| Popis: | Background: A stable isotope tracer method to quantify the synthesis of proteins of hepatic origin in response to feeding is described. The response of albumin synthesis on one mixed meal in a piglet model was investigated and the intragastric and intravenous administration modes of C-13-valine were compared. Methods: The fasting and postprandial fractional synthesis rates (FSRs) of albumin in 15 piglets were measured while infusion rates of C-13-valine were changed in anticipation of the increased appearance of the tracee after a single liquid food bolus (30 mL/kg infant formula). C-13-valine enrichments in albumin hydrolysates at regular time intervals were determined with gas chromatography-combustion isotope ratio mass spectrometry. Results: The intravenous mode (n = 8) showed constant plasma alpha-ketoisovalerate tracer-to-tracee ratios (coefficient of variation range: 1-8%), and a 27% increase in albumin FSR after the food bolus (mean FSR +/- standard error [SE]: fasting 14.4% +/- 1.6% vs. postprandial 18.3% +/- 2.2% per day; P |
| Databáze: | OpenAIRE |
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