Comparing Intracellular Stability and Targeting of Sulfobetaine Quantum Dots with Other Surface Chemistries in Live Cells
Autor: | Muro, E., Fragola, A., Pons, T., Lequeux, N., Ioannou, Androulla, Skourides, Paris A., Dubertret, B. |
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Přispěvatelé: | Skourides, Paris A. [0000-0003-3502-5729] |
Rok vydání: | 2012 |
Předmět: |
Cytoplasm
Embryo Nonmammalian Quantum Dot Nanoparticle Ligands Xenopus laevis chemistry.chemical_compound thioctic acid microinjection Semiconductor quantum dots animal betaine General Materials Science dihydrolipoate Thioctic Acid Chemistry specific staining Electroporation Pinocytosis article Living cell intracellular stability Biotinylation cytoplasm Stability HeLa cell living cells Intracellular Biotechnology electroporation Streptavidin Microinjections Cells quantum dots Nanotechnology Conjugated system chemistry animal embryo Biomaterials sulfobetaine Quantum Dots Animals Humans human technology industry and agriculture Proteins General Chemistry equipment and supplies Surface chemistry Betaine Quantum dot drug derivative Cytology metabolism HeLa Cells |
Zdroj: | Small |
ISSN: | 1613-6810 |
Popis: | The in vivo labeling of intracellular components with quantum dots (QDs) is very limited because of QD aggregation in the cell cytoplasm and/or QD confinement into lysosomal compartments. In order to improve intracellular targeting with QDs, various surface chemistries and delivery methods have been explored, but they have not yet been compared systematically with respect to the QD intracellular stability. In this work, the intracellular aggregation kinetics of QDs for three different surface chemistries based on ligand exchange or encapsulation with amphiphilic polymers are compared. For each surface chemistry, three delivery methods for bringing the nanoparticles into the cells are compared: electroporation, microinjection, and pinocytosis. It is concluded that the QD intracellular aggregation behavior is strongly dependent on the surface chemistry. QDs coated with dihydrolipoic acid-sulfobetaine (DHLA-SB) ligands diffuse freely in cells for longer periods of time than for QDs in the other chemistries tested, and they can access all cytoplasmic compartments. Even when conjugated to streptavidin, these DHLA-SB QDs remain freely diffusing inside the cytoplasm and unaggregated, and they are able to reach a biotinylated target inside HeLa cells. Such labeling was more efficient when compared to commercial streptavidin-conjugated QDs, which may be due to the smaller size of DHLA-SB QDs and/or to their superior intracellular stability. Quantum dots (QDs) solubilized with the zwitterionic ligand dihydrolipoic acid-sulfobetaine present excellent in vivo stability when introduced into cultured cells and embryos. This intracellular stability is much higher than with other QD surface chemistries. When conjugated to streptavidin, these zwitterionic QDs can label an intracellular target with great specificity, higher than commercial streptavidin QDs. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. 8 1029 1037 Cited By :29 |
Databáze: | OpenAIRE |
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