Mobilization of specific maternal RNA species into polysomes after fertilization in Xenopus laevis

Autor:	Eva Dworkin-Rastl, Anthony Shrutkowski, Mark B. Dworkin
Rok vydání:	1985
Předmět:	Genetics Messenger RNA Multidisciplinary Base Sequence biology Embryogenesis Xenopus RNA Embryo biology.organism_classification Cell biology Xenopus laevis Fertilization Polyribosomes Polysome Gene expression Protein biosynthesis Animals RNA Messenger Research Article
Zdroj:	Proceedings of the National Academy of Sciences. 82:7636-7640
ISSN:	1091-6490 0027-8424
DOI:	10.1073/pnas.82.22.7636
Popis:	Unfertilized eggs of many species contain large amounts of maternal mRNA that are used to support protein synthesis during the first few hours of development, before the onset of embryonic transcription. We have examined the accumulation of nonpolysomal maternal RNAs in polysomes after fertilization in Xenopus laevis by measuring the distributions of specific sequences in nonpolysomal and polysomal fractions. In an arbitrary selection of 18 maternal sequences that are largely nonpolysomal in the full-grown oocyte, 13 became enriched in polysomes by the 16-cell cleavage stage. One sequence accumulated only 50% in polysomes at this time, while four sequences became polysomal later than the 16-cell stage. Several RNA sequences decreased in titer during early embryogenesis and were rare during organogenesis. Sequences that are mobilized rapidly and efficiently into polysomes shortly after fertilization and whose cellular concentrations are highest in embryos before organogenesis may provide genetic information for developmental functions restricted to very early embryogenesis. These experiments serve to identify such sequences in Xenopus.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::25acd4fc25b572c0b10a51a1cb218c52 https://doi.org/10.1073/pnas.82.22.7636 Zobrazit plný text záznamu