Increased gene expression and inflammatory cell infiltration caused by electroporation are both important for improving the efficacy of DNA vaccines
| Autor: | Lorne A. Babiuk, Georg Widera, Shawn Babiuk, Dorothy M. Middleton, Maria E. Baca-Estrada, Deitmar Rabussay, Marianna Foldvari |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Time Factors Swine Gene Expression Bioengineering Inflammation Biology Injections Intramuscular Applied Microbiology and Biotechnology DNA vaccination Interferon-gamma Necrosis Viral Proteins Immune system Plasmid Viral Envelope Proteins Downregulation and upregulation Gene expression Leukocytes Vaccines DNA medicine Animals Luciferases Muscle Skeletal Hepatitis B Surface Antigens Electroporation Gene Transfer Techniques General Medicine medicine.disease Up-Regulation Cellular infiltration Immunology Cancer research Female medicine.symptom Plasmids Biotechnology |
| Zdroj: | Journal of Biotechnology. 110:1-10 |
| ISSN: | 0168-1656 |
| DOI: | 10.1016/j.jbiotec.2004.01.015 |
| Popis: | One potential reason for the enhancement of immune responses to DNA vaccines following electroporation is increased gene expression. However, the inflammatory response and accompanying cellular infiltration stimulated by electroporation may also be essential for enhancing immune responses to DNA vaccines. These parameters were investigated in pigs, using different electroporation conditions to induce different levels of gene expression and inflammation. Results indicated that the least effective strategy was conventional intramuscular injection where there was low gene expression and low inflammatory cell infiltration. The most efficacious strategy was plasmid administration immediately followed by electroporation. This latter set of conditions elicited a combination of high gene expression and high cellular infiltration. This indicates that electroporation enhances immune responses to DNA vaccines through increased gene expression and inflammatory cell infiltration. |
| Databáze: | OpenAIRE |
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