Centrioles generate a local pulse of Polo/PLK1 activity to initiate mitotic centrosome assembly
| Autor: | Siu‐Shing Wong, Zachary M Wilmott, Saroj Saurya, Ines Alvarez‐Rodrigo, Felix Y Zhou, Kwai‐Yin Chau, Alain Goriely, Jordan W Raff |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Centrosome
animal structures CDK5RAP2 General Immunology and Microbiology Centriole General Neuroscience Mitosis Cell Cycle Proteins Biology Protein Serine-Threonine Kinases PLK1 General Biochemistry Genetics and Molecular Biology Cell biology enzymes and coenzymes (carbohydrates) Drosophila melanogaster Animals Drosophila Proteins biological phenomena cell phenomena and immunity Mother centriole Molecular Biology Blastoderm Pericentriolar material Centrioles |
| DOI: | 10.15252/embj.2022110891 |
| Popis: | Mitotic centrosomes are formed when centrioles start to recruit large amounts of pericentriolar material (PCM) around themselves in preparation for mitosis. This centrosome “maturation” requires the centrioles and also Polo/PLK1 protein kinase. The PCM comprises several hundred proteins and, in Drosophila, Polo cooperates with the conserved centrosome proteins Spd-2/CEP192 and Cnn/CDK5RAP2 to assemble a PCM scaffold around the mother centriole that then recruits other PCM client proteins. We show here that in Drosophila syncytial blastoderm embryos, centrosomal Polo levels rise and fall during the assembly process—peaking, and then starting to decline, even as levels of the PCM scaffold continue to rise and plateau. Experiments and mathematical modelling indicate that a centriolar pulse of Polo activity, potentially generated by the interaction between Polo and its centriole receptor Ana1 (CEP295 in humans), could explain these unexpected scaffold assembly dynamics. We propose that centrioles generate a local pulse of Polo activity prior to mitotic entry to initiate centrosome maturation, explaining why centrioles and Polo/PLK1 are normally essential for this process. |
| Databáze: | OpenAIRE |
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