Rational design of an ESIPT-based fluorescent probe for selectively monitoring glutathione in live cells and zebrafish
Autor: | Di-Hua Tian, Han-Chen Zhang, De-Chen Duan, Fang Dai, Bo Zhou, Ya-Long Zheng |
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Rok vydání: | 2020 |
Předmět: |
Antioxidant
medicine.medical_treatment Endogeny 02 engineering and technology 010402 general chemistry PC12 Cells 01 natural sciences Analytical Chemistry chemistry.chemical_compound medicine Animals Humans Instrumentation Zebrafish Spectroscopy Fluorescent Dyes biology Optical Imaging Rational design Hep G2 Cells Glutathione 021001 nanoscience & nanotechnology biology.organism_classification Fluorescence Atomic and Molecular Physics and Optics Rats 0104 chemical sciences Spectrometry Fluorescence Benzothiazole chemistry Biophysics 0210 nano-technology Intracellular |
Zdroj: | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 238:118429 |
ISSN: | 1386-1425 |
Popis: | Glutathione (GSH), an extremely important antioxidant, is a major participant in maintaining redox homeostasis and tightly associated with various clinical diseases. Thus, accurate and rapid detection of intracellular GSH is imperative to elucidate its role in physiological and pathological processes. Herein, by modifying 2-(2′-hydroxyphenyl) benzothiazole (HBT) scaffold, we developed an excited-state intramolecular proton transfer (ESIPT)-based fluorescent probe BTFMD for tracking GSH, which exhibited good selectivity, excellent water solubility, a large Stokes shift (181 nm) and fast response rate (within 10 min). Furthermore, the probe was successfully applied for imaging of endogenous GSH in live cells and zebrafish, and probing into the role of GSH in the development of cancer and Parkinson's disease. |
Databáze: | OpenAIRE |
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