| Autor: |
Chalak, Amani, Villares, Ana, Celine Moreau, Haon, Mireille, Grisel, Sacha, Angélina D’Orlando, Herpoël-Gimbert, Isabelle, Labourel, Aurore, Cathala, Bernard, Jean-Guy Berrin |
| Rok vydání: |
2019 |
| DOI: |
10.6084/m9.figshare.9766106 |
| Popis: |
Additional file 1: Figure S1. SDS-PAGE analysis of LPMO-FL and LPMO-CD purified enzymes. Ladder size is indicated in kDa. Figure S2. Time-course analysis and quantification of the Glc4 (plain lines) and Glc3 (dotted lines) released by LPMO-FL (triangles) and LPMO-CD (circles) acting on cellohexaose over a total period of 24 h. Figure S3. Qualitative cellulose binding assays. Binding of LPMO-FL and LPMO-CD to (a) PASC (0.3% (w/v)), (b) NFC (0.3% (w/v)) and (c) BMCC (0.3% (w/v)). Lane 1, control (no substrate); lane 2, unbound material; lane 3, wash 1; lane 4, bound fraction. Experiments were carried out on ice using 30 µg of proteins and 50 mM sodium acetate buffer pH 5.2 in a final volume 200 µL without added l-cysteine. Ladder size is given in kDa. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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