Vitrification of human islets of Langerhans
| Autor: | G.J. Bruining, P. Heyse, Nicolet H. P. M. Jutte, Gerard H. Zeilmaker, H.G. Jansen |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
endocrine system
medicine.medical_specialty endocrine system diseases Cell Survival medicine.medical_treatment Peptide hormone Biology General Biochemistry Genetics and Molecular Biology Cryopreservation Islets of Langerhans Internal medicine Freezing Insulin Secretion medicine Humans Insulin Vitrification geography geography.geographical_feature_category General Medicine Islet In vitro Microscopy Electron Endocrinology Cell culture Tissue Preservation General Agricultural and Biological Sciences Endocrine gland |
| Zdroj: | Cryobiology. 24(5) |
| ISSN: | 0011-2240 |
| Popis: | Cryopreservation of human islets of Langerhans by vitrification was studied. Isolated islets were divided into four groups: (1) control islets which were cultured for 6 days, (2) islets which were vitrified after 2 days of culture, (3) control islets which were cultured for 10–13 days, and (4) islets which were vitrified after 6–9 days of culture. After warming, islets from groups 2 and 4 were cultured for 4 days. The thus treated islets were investigated with respect to insulin secretion in the presence of 2.5 or 25 m M glucose, capacity to survive during postwarming culture, and morphology. The insulin secretion in islets from all groups could be stimulated by an increase of the concentration of glucose from 2.5 to 25 m M . No significant differences were observed between the insulin secretions of the vitrified and control islets or between the islets vitrified after 2 and 6–9 days of culture. It is concluded that human islets of Langerhans cryopreserved by vitrification are functional in vitro . |
| Databáze: | OpenAIRE |
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