Comparative transcriptomics and histopathological analysis of crucian carp infection by atypical Aeromonas salmonicida
Autor: | Yong Zhang, Wang-dong Zhang, Xu Qian, Xiao-dong Ling, Xingxu Zhao, Wan-hong He, Wei-tao Dong, Jixing Liu |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Iron ion homeostasis Carps Spleen Aeromonas salmonicida Adaptive Immunity Aquatic Science Microbiology Pathogenesis Fish Diseases 03 medical and health sciences Enzyme regulator activity Immune system Intestinal mucosa medicine Animals Environmental Chemistry Phylogeny biology 04 agricultural and veterinary sciences General Medicine biology.organism_classification Immunity Innate 030104 developmental biology medicine.anatomical_structure 040102 fisheries Crucian carp 0401 agriculture forestry and fisheries Gram-Negative Bacterial Infections Transcriptome |
Zdroj: | Fish & Shellfish Immunology. 94:294-307 |
ISSN: | 1050-4648 |
DOI: | 10.1016/j.fsi.2019.09.006 |
Popis: | Aeromonas salmonicida is a ubiquitous fish pathogen known to cause furunculosis. With the emergence of new subtypes and the expansion of the host range, it has threatened the health of a variety of marine and freshwater fish, particularly the non-salmonids, manifesting differently from the classical furunculosis. Although there have been reports of infection by atypical strains on the crucian carp, the pathogenesis and tissue pathology remain unclear. In this study, transcriptomics and histopathology were used to analyze the immune response and lesions of crucian carp infected with A. salmonicida. Comparative analysis showed 6579 differentially expressed genes (DEGs) (3428 down-regulated and 3151 up-regulated) were identified on day 5 post-infection (5 dpi). Further annotation and analysis revealed that the DEGs were enriched in enzyme regulator activity, response to oxidative stress, iron ion homeostasis and other functions, and mitogen-activated protein kinase (MAPK), nuclear factor-κB (NF-κB), toll-like receptor (TLR), and nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) etc., and immune-related signaling pathways. Meanwhile, the four C-type lysozyme genes found in all DEGs were significantly up-regulated after infection. In addition, there was severe bleeding on the body of the infected fish. Also, the intestine, liver, spleen, and kidney showed varying degrees of inflammatory damage, especially the goblet cell hyperplasia of intestinal mucosa epithelium and degeneration and necrosis of renal tubular epithelium cells. Additionally, with the increase in pathogen concentration, the cumulative mortality increased, the severity of lesions in the hindgut and head-kidney tissues increased. The relative expression levels of four immune-related genes (TNF-α, IL-1β, IL-11, C-lysozyme) were also significantly upregulated, compared with the control (P |
Databáze: | OpenAIRE |
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