Chi recombination activity in phage lambda decays as a function of genetic distance
| ISSN: | 0016-6731 |
|---|---|
| Přístupová URL adresa: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::25a17fb30bfec5b611161d5dbbd78977 https://pubmed.ncbi.nlm.nih.gov/8582627 |
| Rights: | OPEN |
| Přírůstkové číslo: | edsair.doi.dedup.....25a17fb30bfec5b611161d5dbbd78977 |
| Autor: | Richard S. Myers, Franklin W. Stahl, Mary M. Stahl |
| Rok vydání: | 1995 |
| Předmět: |
Exonuclease
DNA Replication Exodeoxyribonuclease V Recombination hotspot Base pair DNA Single-Stranded Investigations Bacterial Proteins Sequence Homology Nucleic Acid Genetics Escherichia coli RecBCD Recombination Genetic biology Models Genetic DNA replication DNA Helicases Lambda phage biology.organism_classification Molecular biology Bacteriophage lambda Exodeoxyribonucleases Genetic distance DNA Viral biology.protein Recombination |
| Zdroj: | Genetics. 141(3) |
| ISSN: | 0016-6731 |
| Popis: | In Escherichia coli, chi is a recombination hotspot that stimulates RecBCD-dependent exchange at and to one side of itself. chi activity is highest at chi and decreases with distance from chi. The decrease in chi activity may be a simple property of the physical distance over which chi can stimulate recombination. Alternatively, the decay in chi activity with distance may reflect the high likelihood that chi-stimulated recombination occurs in a single chi-proximal act, to the exclusion of additional chi-stimulated exchanges more distal to chi. To test the models, we determined if chi activity decreases as a function of physical distance (i.e., DNA base pairs) or genetic distance (homologous DNA base pairs). Our results indicate that chi activity decays as a function of genetic distance. In addition, we found that the sbcB gene product (exonuclease I, a 3'-->5' ssDNA exonuclease) modulates the distance over which chi can act. In contrast, the recJ gene product (a 5'-->3' ssDNA exonuclease) does not alter the decay of chi activity. |
| Databáze: | OpenAIRE |
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