Functional characterization of the diatom cyclin-dependent kinase A2 as a mitotic regulator reveals plant-like properties in a non-green lineage
| Autor: | Chris Bowler, Lieven De Veylder, Atsuko Tanaka, Wim Vyverman, Marie J. J. Huysman |
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| Rok vydání: | 2015 |
| Předmět: |
Cell division
Molecular Sequence Data Mitosis PROTEIN Plant Science Cell cycle CDKA2 Phaeodactylum tricornutum CHLAMYDOMONAS-REINHARDTII CELL-DIVISION-CYCLE Cyclin-dependent kinase TOMATO FRUIT YEAST Amino Acid Sequence GENOME-WIDE ANALYSIS PHAEODACTYLUM-TRICORNUTUM Phylogeny GENE-EXPRESSION Cytokinesis Diatoms biology Kinase Algal Proteins Biology and Life Sciences Diatom biology.organism_classification Cyclin-Dependent Kinases Cell biology Gene Expression Regulation ARABIDOPSIS-THALIANA biology.protein Interphase Research Article MARINE DIATOMS |
| Zdroj: | BMC PLANT BIOLOGY BMC Plant Biology |
| ISSN: | 1471-2229 |
| DOI: | 10.1186/s12870-015-0469-6 |
| Popis: | Background Cyclin-dependent kinases (CDKs) are crucial regulators of cell cycle progression in eukaryotes. The diatom CDKA2 was originally assigned to the classical A-type CDKs, but its cell cycle phase-specific transcription at the G2-to-M phase transition is typical for plant-specific B-type CDKs. Results Here, we report the functional characterization of CDKA2 from the diatom Phaeodactylum tricornutum. Through a yeast two-hybrid library screen, CDKA2 was found to interact with the G2/M-specific CDK scaffolding factor CKS1. Localization of CDKA2 was found to be nuclear in interphase cells, while in cells undergoing cytokinesis, the signal extended to the cell division plane. In addition, overexpression of CDKA2 induced an overall reduction in the cell growth rate. Expression analysis of cell cycle marker genes in the overexpression lines indicates that this growth reduction is primarily due to a prolongation of the mitotic phase. Conclusions Our study indicates a role for CDKA2 during cell division in diatoms. The functional characterization of a CDK with clear CDKB properties in a non-green organism questions whether the current definition of B-type CDKs being plant-specific might need revision. Electronic supplementary material The online version of this article (doi:10.1186/s12870-015-0469-6) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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