MicroRNAs 145 and 148a Are Upregulated During Congenital Zika Virus Infection
Autor: | Paula Pezzuto, Maria Elisabeth Lopes Moreira, Alessandro L. Gonçalves, Girlene S. Azevedo, Fabio L. Monteiro, Bruno L. Schamber-Reis, Adriana Suely de Oliveira Melo, Fernanda L. De Castro, Daniela P. Cunha, Douglas F. Nixon, Dominic Paquin-Proulx, Renato S. Aguiar, Loraine Campanati, Raphael M D T Gonçalves, Leila Chimeli, Amilcar Tanuri, Marcelo Ribeiro-Alves, Victor Emmanuel Viana Geddes, Zilton Vasconcelos |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Microcephaly Biology Arbovirus Virus lcsh:RC321-571 Zika virus neuroblastoma 03 medical and health sciences Flaviviridae Zika 0302 clinical medicine Downregulation and upregulation Pregnancy Cell Line Tumor microRNA postmortem brain autopsies medicine Humans Pregnancy Complications Infectious lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Neurons Original Paper Zika Virus Infection General Neuroscience Infant Newborn Brain RNA Stillbirth medicine.disease biology.organism_classification Virology Up-Regulation MicroRNAs 030104 developmental biology Gene Expression Regulation miRNAs Female Neurology (clinical) 030217 neurology & neurosurgery |
Zdroj: | ASN NEURO ASN Neuro, Vol 11 (2019) |
ISSN: | 1759-0914 |
Popis: | Zika virus (ZIKV) is an arthropod-borne virus (arbovirus) member of the Flaviviridae family, which has been associated with the development of the congenital Zika syndrome (CZS). RNA viruses, such as flaviviruses, have been reported to exert a profound impact on host microRNAs (miRNAs). Cellular miRNAs modulated by ZIKV may help identify cellular pathways of relevance to pathogenesis. Here, we screened 754 human cellular miRNAs modulated by ZIKV infection (Brazilian PE strain) in a neuroblastoma cell line. Seven miRNAs (miR-99a*, miR-126*, miR-190b, miR-361-3p, miR-522-3p, miR-299-5p, and miR-1267) were downregulated during ZIKV infection, while miR-145 was upregulated. Furthermore, 11 miRNAs were exclusively expressed in ZIKV-infected (miR-148a, miR-342-5p, miR-598, and miR-708-3p) or mock cells (miR-208, miR-329, miR-432-5p, miR-488, miR-518b, miR-520g, and miR-767-5p). Furthermore, in silico analysis indicated that some central nervous system, cellular migration, and adhesion function-related biological processes were overrepresented in the list of target genes of the miRNAs regulated in ZIKV-infected cells, especially for miR-145 and miR-148a. The induction of miR-145 and miR-148a was confirmed in postmortem brain samples from stillborn with severe CZS. Finally, we determined the expression regulation of microcephaly related genes through RNA interference pathway caused by ZIKV directly on neuron cells. |
Databáze: | OpenAIRE |
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