Haloarcula sebkhae sp. nov., an extremely halophilic archaeon from Algerian hypersaline environment
| Autor: | Stéphane Canaan, Sylvie Rebuffat, Hélène Barreteau, Jean Peduzzi, Manon Vandervennet, Alyssa Carré-Mlouka, Vanessa Point, Laura Guédon |
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| Přispěvatelé: | Institut de Biologie Intégrative de la Cellule (I2BC), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Molécules de Communication et Adaptation des Micro-organismes (MCAM), Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'ingénierie des systèmes macromoléculaires (LISM), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des symbioses tropicales et méditerranéennes (UMR LSTM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Aix Marseille Université (AMU), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine hypersaline environments [SDV]Life Sciences [q-bio] 030106 microbiology [SDV.BID]Life Sciences [q-bio]/Biodiversity 010603 evolutionary biology 01 natural sciences Microbiology sebkha 03 medical and health sciences chemistry.chemical_compound RNA Ribosomal 16S Saline Waters Ecology Evolution Behavior and Systematics Phospholipids Phylogeny haloarchaea Phosphatidylglycerol Growth medium Base Composition Haloarcula biology Strain (chemistry) Pigmentation Fatty Acids Nucleic Acid Hybridization Vitamin K 2 General Medicine Sequence Analysis DNA biology.organism_classification 16S ribosomal RNA rpoB Halophile Lakes DNA Archaeal chemistry Biochemistry 13. Climate action Algeria Haloarchaea salt lake halophilic archaeon |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, 2019, 69 (3), ⟨10.1099/ijsem.0.003211⟩ International Journal of Systematic and Evolutionary Microbiology, 2019, 69 (3), ⟨10.1099/ijsem.0.003211⟩ |
| ISSN: | 1466-5026 1466-5034 |
| DOI: | 10.1099/ijsem.0.003211⟩ |
| Popis: | International audience; A halophilic organism, SWO25T, was isolated from water sampled in Algeria at the salt lake (sebkha) of Ouargla. The novel strain stained Gram-negative, and cells were pleomorphic with a red pigmentation. Strain SWO25T grew optimally at 35–45 °C, at pH 6.0–8.0 and 0.05–0.25 M MgCl2 concentrations. Cells were extremely halophilic, with optimal growth at 4.3–5.1 M NaCl. The predominant membrane polar lipids were C20C20 glycerol diether derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate, phosphatidylglycerol sulfate, triglycosyl diether and diglycosyl diether. The major respiratory menaquinone component was MK-8. Cells were highly tolerant to the presence of decane and isooctane in the growth medium. Chemotaxonomic properties supported the assignment of strain SWO25T to the genus Haloarcula. The DNA G+C content was 61.1mol%. DNA–DNA hybridization and phylogenetic analyses of the 16S rRNA and rpoB′ genes showed that strain SWO25T is distinct from known Haloarcula species. Based on phenotypic, chemotaxonomic, genotypic and phylogenetic data, we describe a novel species of the genus Haloarcula, for which the name Haloarculasebkhae sp. nov. is proposed. The type strain is SWO25T (=CIP 110583T=JCM 19018T). |
| Databáze: | OpenAIRE |
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