No alteration in NK function or ζ chain expression in NK and T cells of cervical cancer patients
| Autor: | Peter L. Stern, Henry C Kitchener, Margaret F Duggan-Keen, Toli S Onon |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Cytotoxicity
Immunologic CD3 Complex T-Lymphocytes Cell Uterine Cervical Neoplasms Human leukocyte antigen Natural killer cell Flow cytometry Interferon-gamma Interleukin 21 Interferon Humans Cytotoxic T cell Medicine Neoplasm Staging Lymphokine-activated killer cell medicine.diagnostic_test business.industry Receptors IgG Age Factors Obstetrics and Gynecology Middle Aged Flow Cytometry Chromium Radioisotopes Killer Cells Natural medicine.anatomical_structure Oncology Immunology Cancer research Female K562 Cells business medicine.drug |
| Zdroj: | Gynecologic Oncology. 89:120-128 |
| ISSN: | 0090-8258 |
| DOI: | 10.1016/s0090-8258(03)00009-x |
| Popis: | Objectives To investigate in vitro natural killer (NK) cell activity and expression of signal-transducing ζ chains in patients with cervical cancer. Patients and methods Experiments were performed with frozen lymphocytes from patients at all disease stages and from healthy controls. Thawed NK were activated by overnight incubation in interferon-γ (IFN-γ); activity against two target cell lines was assessed by 4-h 51 Cr release assay. Targets chosen were K562, an erythroleukemic cell line, and a cervical carcinoma cell line designated 808. T and NK cell ζ chain expression was measured by flow cytometry. Results Patients' NK were found to be as cytotoxic as those of normal controls against cell lines K562 and 808. Patient T and NK cells did not show significant down-regulation of the ζ chain. Conclusions We have found no evidence to suggest that loss of ζ chains is a mechanism for immunocompromise in patients with cervical carcinoma. IFN-recoverable patient NK activity is not reduced compared to matched controls. This may be clinically relevant since NK are active against cells exhibiting class I human leukocyte antigen (HLA) down-regulation and many cervical cancers show loss of HLA. |
| Databáze: | OpenAIRE |
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