Long noncoding RNA FTX is upregulated in gliomas and promotes proliferation and invasion of glioma cells by negatively regulating miR-342-3p
| Autor: | Pengwei Wang, Fei Peng, Hui Li, Jianhua Li, Chen Xie, Weiguang Zhang, Weiwei Liang, Fubin Ren, Dan Zhu, Zhi Wang, Yunke Bi, Laizang Wang, Chenguang Li |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Pathology medicine.medical_specialty Blotting Western Transplantation Heterologous Mice Nude Biology Pathology and Forensic Medicine 03 medical and health sciences Downregulation and upregulation Cell Movement RNA interference Cell Line Tumor Glioma medicine Animals Humans Neoplasm Invasiveness Molecular Biology Cell Proliferation Regulation of gene expression Mice Inbred BALB C Gene knockdown Reverse Transcriptase Polymerase Chain Reaction HEK 293 cells Membrane Proteins RNA-Binding Proteins Cell Biology medicine.disease Long non-coding RNA Tumor Burden Up-Regulation Gene Expression Regulation Neoplastic Transplantation MicroRNAs HEK293 Cells 030104 developmental biology Cancer research RNA Interference RNA Long Noncoding Cell Adhesion Molecules |
| Zdroj: | Laboratory Investigation. 97:447-457 |
| ISSN: | 0023-6837 |
| Popis: | Gliomas remain a major public health challenge, posing a high risk for brain tumor-related morbidity and mortality. However, the mechanisms that drive the development of gliomas remain largely unknown. Emerging evidence has shown that long noncoding RNAs are key factors in glioma pathogenesis. qRT-PCR analysis was used to assess the expression of FTX and miR-342-3p in the different stages of gliomas in tissues. Bioinformatics tool DIANA and TargetSCan were used to predict the targets of FTX and miR-342-3p, respectively. Pearson's correlation analysis was performed to test the correlation between the expression levels of FTX, miR-342-3p, and astrocyte-elevated gene-1 (AEG-1). To examine the role of FTX in regulating proliferation and invasion of glioma cells, specific siRNA was used to knockdown FTX, and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and transwell assays were performed. Furthermore, rescue experiments were performed to further confirm the regulation of miR-342-3p by FTX. We then found that the expression of FTX and miR-342-3p was associated with progression of gliomas. FTX directly inhibited the expression of miR-342-3p, which subsequently regulates the expression of AEG-1. Collectively, FTX is critical for proliferation and invasion of glioma cells by regulating miR-342-3p and AEG-1. Our findings indicate that FTX and miR-342-3p may serve as a biomarker of glioma diagnosis, and offer potential novel therapeutic targets of treatment of gliomas. |
| Databáze: | OpenAIRE |
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