Alternative Internal Standard Calibration of an Indirect Enzymatic Analytical Method for 2-MCPD Fatty Acid Esters
| Autor: | Kousuke Abe, Yukinari Takahashi, Kinuko Miyazaki, Kazuo Koyama, Hidenori Nohara, Toru Nezu, Toru Fukazawa, Hiroshi Yada, Takashi Sano, Yomi Watanabe, Kumiko Yamazaki, Yoshitsugu Egawa, Tadashi Kitta, Takashi Miyashita, Hideji Taniguchi |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Glycerol Propanols General Chemical Engineering Relative standard deviation 03 medical and health sciences chemistry.chemical_compound Dietary Fats Unsaturated Edible oil Calibration Analysis method Enzyme Assays chemistry.chemical_classification Alternative methods 030109 nutrition & dietetics Chromatography Fatty Acids Glycidol Fatty acid Regression analysis Esters General Medicine General Chemistry chemistry Epoxy Compounds Regression Analysis Food Analysis |
| Zdroj: | Journal of oleo science. 66(6) |
| ISSN: | 1347-3352 |
| Popis: | An indirect enzymatic analysis method for the quantification of fatty acid esters of 2-/3-monochloro-1,2-propanediol (2/3-MCPD) and glycidol was developed, using the deuterated internal standard of each free-form component. A statistical method for calibration and quantification of 2-MCPD-d5, which is difficult to obtain, is substituted by 3-MCPD-d5 used for calculation of 3-MCPD. Using data from a previous collaborative study, the current method for the determination of 2-MCPD content using 2-MCPD-d5 was compared to three alternative new methods using 3-MCPD-d5. The regression analysis showed that the alternative methods were unbiased compared to the current method. The relative standard deviation (RSDR) among the testing laboratories was ≤ 15% and the Horwitz ratio was ≤ 1.0, a satisfactory value. |
| Databáze: | OpenAIRE |
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