Outer surface lipoproteins ofBorrelia burgdorferi vary in their ability to induce experimental joint injury
| Autor: | Stephen Batsford, John J. Dunn, Michael J. Mihatsch |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Lipopolysaccharides
Male Lipopolysaccharide Lipoproteins Immunology Arthritis Spirochaetaceae Lyme Arthritis chemistry.chemical_compound Rheumatology medicine Animals Immunology and Allergy Pharmacology (medical) Rats Wistar Borrelia burgdorferi Lyme Disease biology Lipopeptide bacterial infections and mycoses biology.organism_classification medicine.disease Arthritis Experimental Peptide Fragments Rats chemistry Joints lipids (amino acids peptides and proteins) Lipid modification Bacterial Outer Membrane Proteins Lipoprotein |
| Zdroj: | Arthritis & Rheumatism. 50:2360-2369 |
| ISSN: | 1529-0131 0004-3591 |
| DOI: | 10.1002/art.20337 |
| Popis: | Objective To examine the ability of bacterial lipoproteins from the spirochete Borrelia burgdorferi to cause in vivo tissue injury (arthritis). Methods Outer surface proteins (OSPs) from B burgdorferi were used in a rat model of antigen-induced allergic arthritis. Intraarticular challenge with recombinant OspA, OspB, and OspC in nonlipidated (peptide) and lipidated forms was performed in the left knee joint; the contralateral joint received buffer as control. Inflammation was monitored by technetium scintigraphy and histology. Results Nonlipidated (peptide) OspA, OspB, and OspC did not induce arthritis; the only exception was polymerized OspA, which was tested in preimmunized rats. Lipidated OspA from 2 different strains and lipidated OspC induced severe arthritis, whereas lipidated OspB failed to induce injury. A synthetic analog of the OSP lipid modification, lipopeptide Pam3Cys-Ser-Lys4-OH, either alone or coupled to bovine serum albumin, also failed to induce injury. Injury did not develop in control groups that were given the appropriate buffers or lipopolysaccharide. This showed that lipidated borrelial OSPs can be potent arthritogens but vary greatly with respect to their injury-inducing potential. The possession of a lipid modification is essential but is not sufficient to render an OSP arthritogenic. Conclusion This is the first study to demonstrate that individual lipoproteins from B burgdorferi can induce experimental joint injury in vivo. These results may help elucidate the pathogenesis of Lyme arthritis and, above all, underline the importance of bacterial lipoproteins as major virulence factors. |
| Databáze: | OpenAIRE |
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