Microarray-based analysis of fish egg quality after natural or controlled ovulation

Autor: Julien Bobe, Emilie Bonnet, Alexis Fostier
Přispěvatelé: Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA)
Jazyk: angličtina
Rok vydání: 2007
Předmět:
Microarray
medicine.medical_treatment
reproduction animale
Transcriptome
oeuf de poisson
poisson
Biologie de la reproduction
media_common
Genetics
salmonidae
0303 health sciences
Reproductive Biology
oncorhynchus mykiss
Reverse Transcriptase Polymerase Chain Reaction
04 agricultural and veterinary sciences
induction de ponte
Female
DNA microarray
arn messager
Biotechnology
Research Article
Quality Control
lcsh:QH426-470
qualité des oeufs
lcsh:Biotechnology
media_common.quotation_subject
Molecular Sequence Data
Biology
Andrology
03 medical and health sciences
Ovulation Induction
lcsh:TP248.13-248.65
medicine
Animals
Amino Acid Sequence
RNA
Messenger
Ovulation
Gene
030304 developmental biology
Ovum
Sequence Homology
Amino Acid
Microarray analysis techniques
Embryogenesis
[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology
Microarray Analysis
photopériode
lcsh:Genetics
ovulation
040102 fisheries
0401 agriculture
forestry
and fisheries
Ovulation induction
transcriptome
truite arc en ciel
Zdroj: BMC Genomics (8:55), 1-17. (2007)
BMC Genomics
BMC Genomics, BioMed Central, 2007, 8:55, pp.1-17. ⟨10.1186/1471-2164-8-55⟩
BMC Genomics, Vol 8, Iss 1, p 55 (2007)
ISSN: 1471-2164
DOI: 10.1186/1471-2164-8-55⟩
Popis: Background The preservation of fish egg quality after ovulation-control protocols is a major issue for the development of specific biotechnological processes (e.g. nuclear transfer). Depending on the species, it is often necessary to control the timing of ovulation or induce the ovulatory process. The hormonal or photoperiodic control of ovulation can induce specific egg quality defects that have been thoroughly studied. In contrast, the impact on the egg transcriptome as a result of these manipulations has received far less attention. Furthermore, the relationship between the mRNA abundance of maternally-inherited mRNAs and the developmental potential of the egg has never benefited from genome-wide studies. Thus, the present study aimed at studying the rainbow trout (Oncorhynchus mykiss) egg transcriptome after natural or controlled ovulation using 9152-cDNA microarrays. Results The analysis of egg transcriptome after natural or controlled ovulation led to the identification of 26 genes. The expression patterns of 17 of those genes were monitored by real-time PCR. We observed that the control of ovulation by both hormonal induction and photoperiod manipulation induced significant changes in the egg mRNA abundance of specific genes. A dramatic increase of Apolipoprotein C1 (APOC1) and tyrosine protein kinase HCK was observed in the eggs when a hormonal induction of ovulation was performed. In addition, both microarray and real-time PCR analyses showed that prohibitin 2 (PHB2) egg mRNA abundance was negatively correlated with developmental success. Conclusion First, we showed, for the first time in fish, that the control of ovulation using either a hormonal induction or a manipulated photoperiod can induce differences in the egg mRNA abundance of specific genes. While the impact of these modifications on subsequent embryonic development is unknown, our observations clearly show that the egg transcriptome is affected by an artificial induction of ovulation. Second, we showed that the egg mRNA abundance of prohibitin 2 was reflective of the developmental potential of the egg. Finally, the identity and ontology of identified genes provided significant hints that could result in a better understanding of the mechanisms associated with each type of ovulation control (i.e. hormonal, photoperiodic), and in the identification of conserved mechanisms triggering the loss of egg developmental potential.
Databáze: OpenAIRE
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