Cytokine modulation by PX differently affects specific acute phase proteins during sepsis in rats
| Autor: | Benoît Ruot, F. Rambourdin, L. Voisin, Christiane Obled, Cécile Rallière, Michel Dalle, Denis Breuille |
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| Přispěvatelé: | Unité de nutrition et métabolisme protéique, Institut National de la Recherche Agronomique (INRA), Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Nestlé, Nestlé S.A. |
| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: |
Male
interleukine infection expérimentale Physiology medicine.medical_treatment régulation physiologique rattus rattus Pentoxifylline Rats Sprague-Dawley 0302 clinical medicine cytokine Escherichia coli Infections bactérie 0303 health sciences biology Acute-phase protein Free Radical Scavengers 3. Good health Cytokine 030220 oncology & carcinogenesis Tumor necrosis factor alpha medicine.symptom escherichia coli arn messager medicine.drug medicine.medical_specialty Médecine humaine et pathologie Inflammation Sepsis 03 medical and health sciences In vivo Physiology (medical) Internal medicine medicine Animals albumine Interleukin 6 030304 developmental biology glycoprotéine Interleukin-6 Tumor Necrosis Factor-alpha medicine.disease macroglobuline Rats nécrose Endocrinology biology.protein RAT Human health and pathology fibrinogène expérimentation in vivo [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology Acute-Phase Proteins Interleukin-1 |
| Zdroj: | AJP-Regulatory, Integrative and Comparative Physiology AJP-Regulatory, Integrative and Comparative Physiology, American Physiological Society, 1998, 275 (5), pp.R1412-R1419 American Journal of Physiology. Regulatory, Integrative and Comparative Physiology 5 (275), R1412-R1419. (1998) |
| ISSN: | 0363-6119 1522-1490 |
| Popis: | To explore the regulation of the acute phase response in vivo, the effects of pentoxifylline (PX) treatment (100 mg/kg ip 1 h before infection) were investigated in infected and pair-fed rats 2 and 6 days after an intravenous injection of live bacteria ( Escherichia coli). PX treatment prevented the increase in plasma tumor necrosis factor (TNF)-α (peak 1.5 h after the infection) and resulted in an 84 and 61% inhibition of plasma interleukin (IL)-1β and IL-6, respectively (peaks at 3 h). Plasma corticosterone kinetics were not modified by the treatment. Infection increased α1-acid glycoprotein (AGP), α2-macroglobulin (A2M), and fibrinogen plasma concentrations and decreased albumin levels. PX significantly reduced AGP plasma concentration as early as day 2 in infected animals but reduced A2M and fibrinogen plasma levels only at day 6. The treatment had no effect on the albumin plasma concentration. Hepatic AGP and fibrinogen mRNA levels increased in infected rats, whereas those of A2M were unchanged and those of albumin were decreased. Two days after infection, AGP and fibrinogen mRNA levels were reduced in treated infected animals. PX was ineffective in modifying those of A2M and albumin. These data demonstrate, in vivo, that different acute phase proteins are individually regulated in sepsis. The in vivo effects of PX treatment support the hypothesis that TNF-α plays an important role in the regulation of AGP production, whereas other factors seem to be involved in the regulation of A2M, fibrinogen, and albumin expression. |
| Databáze: | OpenAIRE |
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