Nanostructure-specific X-ray tomography reveals myelin levels, integrity and axon orientations in mouse and human nervous tissue

Autor: Piotr Walczak, Manuel Guizar-Sicairos, Jennifer A. McNab, Marianne Liebi, Mark Augath, Michael Zeineh, Timothy M. Shepherd, Aileen Schroeter, Aleezah Balolia, Lin Yang, Markus Rudin, Marios Georgiadis, Oliver Bunk, Zirui Gao, Els Fieremans, Phillip DiGiacomo, Ivan Rajkovic, Christoph Leuze, Valerio Zerbi, Jiangyang Zhang, Dmitry S. Novikov, Benjamin Ades-Aron, Sung-Lyoung Kim, Jelle Veraart, Choong H. Lee, Thomas M. Weiss, Stefan Sommer, Gergely David, Shirish Chodankar
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Central Nervous System
Male
0301 basic medicine
Myelin biology and repair
General Physics and Astronomy
Imaging techniques
Imaging
Mice
Myelin
0302 clinical medicine
Mouse Spinal Cord
Axon
Myelin Sheath
Mice
Knockout
Multidisciplinary
Chemistry
Brain
SAXS
Magnetic Resonance Imaging
3. Good health
medicine.anatomical_structure
Spinal Cord
Child
Preschool
Female
Tomography
Myelin Proteins
Nervous system
Multiple Sclerosis
Nanostructure
Brain development
Science
Neuroimaging
Proof of Concept Study
Article
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
Scattering
Small Angle
medicine
Animals
Humans
X-Rays
Nervous tissue
fungi
General Chemistry
Axons
Nanostructures
Mice
Inbred C57BL
030104 developmental biology
nervous system
Molecular imaging
Tomography
X-Ray Computed
Neuroscience
030217 neurology & neurosurgery
Zdroj: Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
Nature Communications, 12 (1)
Nature Communications
ISSN: 2041-1723
Popis: Myelin insulates neuronal axons and enables fast signal transmission, constituting a key component of brain development, aging and disease. Yet, myelin-specific imaging of macroscopic samples remains a challenge. Here, we exploit myelin’s nanostructural periodicity, and use small-angle X-ray scattering tensor tomography (SAXS-TT) to simultaneously quantify myelin levels, nanostructural integrity and axon orientations in nervous tissue. Proof-of-principle is demonstrated in whole mouse brain, mouse spinal cord and human white and gray matter samples. Outcomes are validated by 2D/3D histology and compared to MRI measurements sensitive to myelin and axon orientations. Specificity to nanostructure is exemplified by concomitantly imaging different myelin types with distinct periodicities. Finally, we illustrate the method’s sensitivity towards myelin-related diseases by quantifying myelin alterations in dysmyelinated mouse brain. This non-destructive, stain-free molecular imaging approach enables quantitative studies of myelination within and across samples during development, aging, disease and treatment, and is applicable to other ordered biomolecules or nanostructures.
Small-angle X-ray scattering (SAXS) combines the high tissue penetration of X-rays with specificity to periodic nanostructures. The authors use SAXS tensor tomography (SAXS-TT) on intact mouse and human brain tissue samples, to quantify myelin levels and determine myelin integrity, myelinated axon orientation, and fibre tracts non-destructively.
Databáze: OpenAIRE
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